| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
2, 4, KLF1 |
| Specificity: |
This antibody reacts specifically with KLF1 2 4 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human KLF1 |
| Gene ID: |
10661 |
| Swiss Prot: |
Q13351 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Able to activate CD44 and AQP1 promoters, Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene, Furthermore, Is a dual regulator of fetal-to-adult globin switching, This dual activity ensures that, When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation (By similarity), fetal hemoglobin levels are low, in most adults, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes, Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
EKLF |
| Also known as: |
KLF1 2 4 Polyclonal Antibody |
| Other name: |
Anti-KLF1 2 4 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
2, 4, KLF1 |
| Short name: |
2, 4 (Polyclonal) Fluor 594, Anti-KLF1 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
2, 4 (polyclonal), ALEXA Fluor 594, antibody to-Kruppel-like factor 1 (erythroid) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
CDAN4 and EKLF and HBFQTL6 and INLU, DNA-templated and biological process this GO :0030218 and erythrocyte differentiation and biological process this GO :0035162 and embryonic hemopoiesis and biological process this GO :0043249 and erythrocyte maturation and biological process this GO :0044212 and transcription regulatory region DNA binding and molecular function this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048821 and erythrocyte development and biological process this GO :1901653 and cellular response to peptide and biological process, KLF1 and IDBG-31252 and ENSG00000105610 and 10661, KLF1 and IDBG-642908 and ENSBTAG00000006083 and 784295, Klf1 and IDBG-175231 and ENSMUSG00000054191 and 16596, metal ion binding, nuclei, this GO :0000790 and nuclear chromatin and cellular component this GO :0000987 and core promoter proximal region sequence-specific DNA binding and molecular function this GO :0001701 and in utero embryonic development and biological process this GO :0001889 and liver development and biological process this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0005634 and nucleus and cellular component this GO :0006338 and chromatin remodeling and biological process this GO :0006351 and transcription, this GO :0000987 : core promoter proximal region sequence-specific DNA binding, this GO :0000987 : core promoter proximal region sequence-specific DNA binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0044212 : transcription regulatory region DNA binding and also this GO :0046872 : metal ion binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0044212 : transcription regulatory region DNA binding, this GO :0046872 : metal ion binding, Kruppel-like factor 1 (erythroid) |
| Identity: |
6345 |
| Gene: |
KLF1 |
More about : KLF1 |
| Long gene name: |
Kruppel like factor 1 |
| Synonyms gene name: |
Kruppel-like factor 1 (erythroid) |
| Synonyms name: |
erythroid Kruppel-like factor |
| Locus: |
19p13, 13 |
| Discovery year: |
1999-12-14 |
| GenBank acession: |
U37106 |
| Entrez gene record: |
10661 |
| Pubmed identfication: |
8924208 9119377 |
| RefSeq identity: |
NM_006563 |
| Classification: |
Kruppel like factors Zinc fingers C2H2-type |
| Havana BLAST/BLAT: |
OTTHUMG00000180536 |
| Locus Specific Databases: |
LRG_825 |