| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
RNF25 |
| Specificity: |
This antibody reacts specifically with RNF25 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human RNF25 |
| Gene ID: |
64320 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
RNF25 (RING finger protein 25), RNF25 is thought to exhibit E2-dependent E3 ubiquitin-protein ligase activity, RNF25 may also regulate the transcriptional activity of NF-kappa-B, Specifically, The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), Via its RING-type zinc finger (a common domain that contains a conserved cysteine-rich region and is found in a number of viral and eukaryotic proteins), a protein that plays important roles in cell survival, also known as AO7, facilitates the ubiquitination of target proteins, host defense and proliferation, inflammation response, is a 459 amino acid protein that contains one RWD domain and one RING-type zinc finger, the RING finger of RNF25 interacts with an E2 ligase and, the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s), through this interaction, Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
AO7 FLJ13906 |
| Also known as: |
RNF25 Polyclonal Antibody |
| Other name: |
Anti-RNF25 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
RNF25 |
| Short name: |
Anti-RNF25 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-RNF25 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
14662 |
| Gene: |
RNF25 |
More about : RNF25 |
| Long gene name: |
ring finger protein 25 |
| Locus: |
2q35 |
| Discovery year: |
2001-02-21 |
| Entrez gene record: |
64320 |
| Pubmed identfication: |
12748188 |
| RefSeq identity: |
NM_022453 |
| Classification: |
Ring finger proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000133077 |