| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
caspase-9 p10 |
| Specificity: |
This antibody reacts specifically with caspase-9 p10 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Mitochondrion, Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human caspase-9 subunit p10 |
| Gene ID: |
842 |
| Swiss Prot: |
P55211 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3, Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9, Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner, Proteolytically cleaves poly(ADP-ribose) polymerase (PARP), Involved in the activation cascade of caspases responsible for apoptosis execution |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
APAF-3, APAF3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, Caspase-9, ICE-LAP6, ICE-like apoptotic protease 6, PPP1R56, MCH6 |
| Also known as: |
caspase-9 p10 Polyclonal Antibody |
| Other name: |
Anti-caspase-9 p10 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Description: |
CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways, Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
caspase-9 p10 |
| Short name: |
Anti-caspase-9 p10 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-caspase-9 p10 (polyclonal) |
| Alternative technique: |
polyclonals |