| Antigen detectable with this ELISA kit: |
Forkhead Box Protein P3 (FOXP3) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
115ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Forkhead Box Protein P3 (FOXP3), The concentration of Forkhead Box Protein P3 (FOXP3) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Forkhead Box Protein P3 (FOXP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Forkhead Box Protein P3 (FOXP3), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Forkhead Box Protein P3 (FOXP3) |
| Short name: |
Forkhead Box Protein P3 (FOXP3) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Forkhead Box Protein P3 (forkhead box P3) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CD25-positive, CD25-positive, DNA-templated and biological process this GO :0006357 and regulation of transcription from RNA polymerase II promoter and biological process this GO :0006366 and transcription from RNA polymerase II promoter and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0009615 and response to virus and biological process this GO :0010628 and positive regulation of gene expression and biological process this GO :0031064 and negative regulation of histone deacetylation and biological process this GO :0032088 and negative regulation of NF-kappaB transcription factor activity and biological process this GO :0032689 and negative regulation of interferon-gamma production and biological process this GO :0032693 and negative regulation of interleukin-10 production and biological process this GO :0032703 and negative regulation of interleukin-2 production and biological process this GO :0032713 and negative regulation of interleukin-4 production and biological process this GO :0032714 and negative regulation of interleukin-5 production and biological process this GO :0032715 and negative regulation of interleukin-6 production and biological process this GO :0032720 and negative regulation of tumor necrosis factor production and biological process this GO :0032753 and positive regulation of interleukin-4 production and biological process this GO :0032792 and negative regulation of CREB transcription factor activity and biological process this GO :0032831 and positive regulation of CD4-positive, DNA-templated and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046007 and negative regulation of activated T cell proliferation and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048294 and negative regulation of isotype switching to IgE isotypes and biological process this GO :0048302 and regulation of isotype switching to IgG isotypes and biological process this GO :0050672 and negative regulation of lymphocyte proliferation and biological process this GO :0050710 and negative regulation of cytokine secretion and biological process this GO :0050728 and negative regulation of inflammatory response and biological process this GO :0050777 and negative regulation of immune response and biological process this GO :0050852 and T cell receptor signaling pathway and biological process this GO :0051059 and NF-kappaB binding and molecular function this GO :0051525 and NFAT protein binding and molecular function, FOXP3 and IDBG-637186 and ENSBTAG00000013279 and 506053, FOXP3 and IDBG-66215 and ENSG00000049768 and 50943, Foxp3 and IDBG-128986 and ENSMUSG00000039521 and 20371, NFAT protein binding, alpha-beta regulatory T cell differentiation and biological process this GO :0002456 and T cell mediated immunity and biological process this GO :0002507 and tolerance induction and biological process this GO :0002513 and tolerance induction to self antigen and biological process this GO :0002637 and regulation of immunoglobulin production and biological process this GO :0002666 and positive regulation of T cell tolerance induction and biological process this GO :0002669 and positive regulation of T cell anergy and biological process this GO :0002677 and negative regulation of chronic inflammatory response and biological process this GO :0002725 and negative regulation of T cell cytokine production and biological process this GO :0002851 and positive regulation of peripheral T cell tolerance induction and biological process this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0003705 and RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity and molecular function this GO :0003714 and transcription corepressor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006338 and chromatin remodeling and biological process this GO :0006355 and regulation of transcription, alpha-beta regulatory T cell differentiation and biological process this GO :0032914 and positive regulation of transforming growth factor beta1 production and biological process this GO :0033092 and positive regulation of immature T cell proliferation in thymus and biological process this GO :0035035 and histone acetyltransferase binding and molecular function this GO :0035066 and positive regulation of histone acetylation and biological process this GO :0035067 and negative regulation of histone acetylation and biological process this GO :0042036 and negative regulation of cytokine biosynthetic process and biological process this GO :0042110 and T cell activation and biological process this GO :0042130 and negative regulation of T cell proliferation and biological process this GO :0042803 and protein homodimerization activity and molecular function this GO :0042826 and histone deacetylase binding and molecular function this GO :0043029 and T cell homeostasis and biological process this GO :0043234 and protein complex and cellular component this GO :0043433 and negative regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0045066 and regulatory T cell differentiation and biological process this GO :0045077 and negative regulation of interferon-gamma biosynthetic process and biological process this GO :0045085 and negative regulation of interleukin-2 biosynthetic process and biological process this GO :0045591 and positive regulation of regulatory T cell differentiation and biological process this GO :0045892 and negative regulation of transcription, nuclei, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0001782 and B cell homeostasis and biological process this GO :0001816 and cytokine production and biological process this GO :0002262 and myeloid cell homeostasis and biological process this GO :0002361 and CD4-positive, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0003705 : RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity and also this GO :0003714 : transcription corepressor activity and also this GO :0005515 : protein binding and also this GO :0035035 : histone acetyltransferase binding and also this GO :0042803 : protein homodimerization activity and also this GO :0042826 : histone deacetylase binding and also this GO :0043565 : sequence-specific DNA binding and also this GO :0046872 : metal ion binding and also this GO :0051059 : NF-kappaB binding and also this GO :0051525 : NFAT protein binding, this GO :0003705 : RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, this GO :0003714 : transcription corepressor activity, this GO :0005515 : protein binding, this GO :0035035 : histone acetyltransferase binding, this GO :0042803 : protein homodimerization activity, this GO :0042826 : histone deacetylase binding, this GO :0043565 : sequence-specific DNA binding, this GO :0046872 : metal ion binding, this GO :0051059 : NF-kappaB binding, this GO :0051525 : NFAT protein binding, forkhead box P3 |
| Identity: |
6106 |
| Gene: |
FOXP3 |
More about : FOXP3 |
| Long gene name: |
forkhead box P3 |
| Synonyms gene: |
IPEX |
| Synonyms gene name: |
X-linked , enteropathy, immune dysregulation, polyendocrinopathy |
| Synonyms: |
JM2 XPID AIID PIDX DIETER SCURFIN |
| Locus: |
Xp11, 23 |
| Discovery year: |
2000-05-05 |
| Entrez gene record: |
50943 |
| Pubmed identfication: |
10677306 11138001 |
| RefSeq identity: |
NM_014009 |
| Classification: |
Forkhead boxes |
| Havana BLAST/BLAT: |
OTTHUMG00000024135 |
| Locus Specific Databases: |
FOXP3base: Mutation registry for Immunodysregulation, IPEX Mental Retardation database LRG_62 , X-linked, and enteropathy, polyendocrinopathy |