| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Centromere protein K |
| Specificity: |
This antibody reacts specifically with Centromere protein K |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human FKSG14/Centromere protein K |
| Gene ID: |
64105 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Acts in coordination with CASC5/KNL1 to recruit the NDC80 complex to the outer kinetochore, FKSG14 constitutively localized to centromeres throughout the cell cycle, It may be involved in incorporation of CENPA into centromeres and is required for proper kinetochore function, May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex, There are 3 isoforms produced by alternative splicing, also known as CENPK (centromere protein K) is a component of the CENPA-CAD (nucleosome distal) complex, mitotic progression and chromosome segregation, FKSG14 |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
FKSG14 SOLT CENP-K |
| Also known as: |
Centromere protein K Polyclonal Antibody |
| Other name: |
Anti-Centromere protein K Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Centromere protein K |
| Short name: |
Anti-Centromere protein K (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Centromere protein K (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
29479 |
| Gene: |
CENPK |
More about : CENPK |
| Long gene name: |
centromere protein K |
| Locus: |
5q12, 3 |
| Discovery year: |
2006-06-15 |
| GenBank acession: |
BC008504 |
| Entrez gene record: |
64105 |
| Pubmed identfication: |
8950979 |
| RefSeq identity: |
NM_022145 |
| Havana BLAST/BLAT: |
OTTHUMG00000131227 |