| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Ube2H |
| Specificity: |
This antibody reacts specifically with Ube2H |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Ube2H/UBCH2 |
| Gene ID: |
7328 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Rat (Rattus norvegicus), Mouse (Mus musculus) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Multiple isoforms of UBE2H exist due to alternative splicing events, One of several members of the ubiquitin-conjugating enzyme family, The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), UBCH, UBCH2 or E2-20K, UBE2H functions as an E2 ubiquitin-conjugating enzyme that acts to catalyze the covalent attachment of ubiquitin residues to various proteins, UBE2H shares 100% identity with its mouse counterpart and 98% identity with its frog and zebrafish homologs, Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction, also known as UBC8, including Histone H2A, is a 183 amino acid protein involved in ubiquitin-mediated protein degradation, suggesting a conserved function between species, the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s), UBE2H |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
UBCH UBC8 GID3 |
| Also known as: |
Ube2H Polyclonal Antibody |
| Other name: |
Anti-Ube2H Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Ube2H |
| Short name: |
Anti-Ube2H (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Ube2H (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
12484 |
| Gene: |
UBE2H |
More about : UBE2H |
| Long gene name: |
ubiquitin conjugating enzyme E2 H |
| Synonyms gene name: |
ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8) ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) ubiquitin-conjugating enzyme E2H |
| Synonyms name: |
GID complex subunit 3, UBC8 homolog (S, cerevisiae) |
| Locus: |
7q32, 2 |
| Discovery year: |
1995-07-12 |
| GenBank acession: |
BC006277 |
| Entrez gene record: |
7328 |
| Pubmed identfication: |
8132613 |
| RefSeq identity: |
NM_003344 |
| Classification: |
Ubiquitin conjugating enzymes E2 |
| Havana BLAST/BLAT: |
OTTHUMG00000157650 |