| Antigen detectable with this ELISA kit: |
Flotillin 2 (FLOT2) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 1, 10ng/mL, 2, 20ng/mL, 5ng/mL, 25ng/mL, 5ng/mL, 625-40ng/mL The standard curve concentrations used for the ELISA&rsquo, 625ng/mL, s were 40ng/mL |
| Sensitivity: |
216ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Flotillin 2 (FLOT2), The concentration of Flotillin 2 (FLOT2) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Flotillin 2 (FLOT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Flotillin 2 (FLOT2), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Flotillin 2 (FLOT2) |
| Short name: |
Flotillin 2 (FLOT2) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Flotillin 2 (flotillin 2) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
Cell surfaces, ECS-1 and ECS1 and ESA and ESA1 and M17S1, FLOT2 and IDBG-37810 and ENSG00000132589 and 2319, FLOT2 and IDBG-632132 and ENSBTAG00000010073 and 615679, Flot2 and IDBG-202091 and ENSMUSG00000061981 and 14252, ionotropic glutamate receptor binding, this GO :0002020 and protease binding and molecular function this GO :0002080 and acrosomal membrane and cellular component this GO :0005515 and protein binding and molecular function this GO :0005768 and endosome and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005901 and caveola and cellular component this GO :0007155 and cell adhesion and biological process this GO :0008544 and epidermis development and biological process this GO :0010629 and negative regulation of gene expression and biological process this GO :0016020 and membrane and cellular component this GO :0016600 and flotillin complex and cellular component this GO :0030139 and endocytic vesicle and cellular component this GO :0031982 and vesicle and cellular component this GO :0035255 and ionotropic glutamate receptor binding and molecular function this GO :0045121 and membrane raft and cellular component this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0050821 and protein stabilization and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0090002 and establishment of protein localization to plasma membrane and biological process this GO :1902992 and negative regulation of amyloid precursor protein catabolic process and biological process, this GO :0002020 : protease binding, this GO :0002020 : protease binding and also this GO :0005515 : protein binding and also this GO :0035255 : ionotropic glutamate receptor binding, this GO :0005515 : protein binding, this GO :0035255 : ionotropic glutamate receptor binding, flotillin 2 |
| Identity: |
3758 |
| Gene: |
FLOT2 |
More about : FLOT2 |
| Long gene name: |
flotillin 2 |
| Synonyms gene: |
M17S1 |
| Synonyms: |
ESA ESA1 ECS-1 ECS1 |
| Synonyms name: |
Flotillin 2 (epidermal surface antigen 1) membrane component, chromosome 17, surface marker 1 (35kD protein identified by monoclonal antibody ECS-1) |
| Locus: |
17q11, 2 |
| Discovery year: |
1991-05-09 |
| GenBank acession: |
M60922 |
| Entrez gene record: |
2319 |
| Pubmed identfication: |
1769667 |
| RefSeq identity: |
NM_004475 |
| Classification: |
Flotillins |
| Havana BLAST/BLAT: |
OTTHUMG00000132674 |