| Antigen detectable with this ELISA kit: |
FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
125ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP), The concentration of FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) |
| Short name: |
FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Identity: |
3942 |
| Gene: |
MTOR |
More about : MTOR |
| Long gene name: |
mechanistic target of rapamycin |
| Synonyms gene: |
FRAP FRAP2 FRAP1 |
| Synonyms gene name: |
FK506 binding protein 12-rapamycin associated protein 1 mechanistic target of rapamycin (serine/threonine kinase) |
| Synonyms: |
RAFT1 RAPT1 FLJ44809 |
| Synonyms name: |
FK506 binding protein 12-rapamycin associated protein 2 rapamycin target protein FKBP12-rapamycin complex-associated protein 1 FKBP-rapamycin associated protein rapamycin associated protein FRAP2 dJ576K7, 1 (FK506 binding protein 12-rapamycin associated protein 1) rapamycin and FKBP12 target 1 mammalian target of rapamycin |
| Locus: |
1p36, 22 |
| Discovery year: |
1995-07-18 |
| GenBank acession: |
L34075 |
| Entrez gene record: |
2475 |
| Pubmed identfication: |
8008069 8660990 |
| RefSeq identity: |
NM_004958 |
| Classification: |
MTOR complex 1 MTOR complex 2 |
| Havana BLAST/BLAT: |
OTTHUMG00000002001 |
| Locus Specific Databases: |
LRG_734 |