| Antigen detectable with this ELISA kit: |
Filamin Binding LIM Protein 1 (FBLIM1) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
058ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Filamin Binding LIM Protein 1 (FBLIM1), The concentration of Filamin Binding LIM Protein 1 (FBLIM1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filamin Binding LIM Protein 1 (FBLIM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Filamin Binding LIM Protein 1 (FBLIM1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Filamin Binding LIM Protein 1 (FBLIM1) |
| Short name: |
Filamin Binding LIM Protein 1 (FBLIM1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Filamin Binding LIM Protein 1 (filamin binding LIM protein 1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
Cell surfaces, FBLIM1 and IDBG-635735 and ENSBTAG00000018725 and 767833, FBLIM1 and IDBG-91122 and ENSG00000162458 and 54751, Fblim1 and IDBG-202206 and ENSMUSG00000006219 and 74202, filamin binding, this GO :0001725 and stress fiber and cellular component this GO :0005829 and cytosol and cellular component this GO :0005925 and focal adhesion and cellular component this GO :0005938 and cell cortex and cellular component this GO :0008270 and zinc ion binding and molecular function this GO :0008360 and regulation of cell shape and biological process this GO :0016337 and single organismal cell-cell adhesion and biological process this GO :0031005 and filamin binding and molecular function this GO :0033623 and regulation of integrin activation and biological process this GO :0034329 and cell junction assembly and biological process, this GO :0008270 : zinc ion binding, this GO :0008270 : zinc ion binding and also this GO :0031005 : filamin binding, this GO :0031005 : filamin binding, filamin binding LIM protein 1 |
| Identity: |
24686 |
| Gene: |
FBLIM1 |
More about : FBLIM1 |
| Long gene name: |
filamin binding LIM protein 1 |
| Synonyms: |
FBLP-1 CAL migfilin |
| Locus: |
1p36, 21 |
| Discovery year: |
2005-06-15 |
| Entrez gene record: |
54751 |
| Pubmed identfication: |
12679033 12496242 |
| RefSeq identity: |
NM_001024215 |
| Classification: |
LIM domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000003079 |