| Antigen detectable with this ELISA kit: |
Acidic (FGF1), Fibroblast Growth Factor 1 |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
125pg/mL, 15, 250pg/mL, 31, 500pg/mL, 15, 62, 25pg/mL, 5pg/mL, 625-1000pg/mL The standard curve concentrations used for the ELISA&rsquo, 625pg/mL, s were 1000pg/mL |
| Sensitivity: |
7pg/mL, The minimum detectable dose of this kit is typically less than 5 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell culture supernates and other biological fluids, cell lysates, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, Acidic (FGF1), Acidic (FGF1), Acidic (FGF1), Acidic (FGF1) in the samples is then determined by comparing the O, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 1, The concentration of Fibroblast Growth Factor 1, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Fibroblast Growth Factor 1, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Acidic (FGF1), Fibroblast Growth Factor 1 |
| Short name: |
Acidic (FGF1) ELISA kit, Fibroblast Growth Factor 1 |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Acidic (fibroblast growth factor 1 (acidic)) Enzyme-linked immunosorbent assay reagent, Fibroblast Growth Factor 1 |
| Alternative technique: |
elisas |
| Alternative to gene target: |
AFGF and ECGF and ECGF-beta and ECGFA and ECGFB and FGF-1 and FGF-alpha and FGFA and GLIO703 and HBGF-1 and HBGF1, FGF1 and IDBG-51235 and ENSG00000113578 and 2246, FGF1 and IDBG-646137 and ENSBTAG00000005198 and 281160, Fgf1 and IDBG-141787 and ENSMUSG00000036585 and 14164, S100 protein binding, nuclei, this GO :0001525 and angiogenesis and biological process this GO :0001759 and organ induction and biological process this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0005104 and fibroblast growth factor receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005578 and proteinaceous extracellular matrix and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005634 and nucleus and cellular component this GO :0005730 and nucleolus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005938 and cell cortex and cellular component this GO :0007165 and signal transduction and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0008083 and growth factor activity and molecular function this GO :0008201 and heparin binding and molecular function this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008286 and insulin receptor signaling pathway and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0009653 and anatomical structure morphogenesis and biological process this GO :0030324 and lung development and biological process this GO :0030335 and positive regulation of cell migration and biological process this GO :0030544 and Hsp70 protein binding and molecular function this GO :0034605 and cellular response to heat and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0044548 and S100 protein binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045542 and positive regulation of cholesterol biosynthetic process and biological process this GO :0045766 and positive regulation of angiogenesis and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048015 and phosphatidylinositol-mediated signaling and biological process this GO :0050679 and positive regulation of epithelial cell proliferation and biological process this GO :0051781 and positive regulation of cell division and biological process this GO :0060681 and branch elongation involved in ureteric bud branching and biological process this GO :0072163 and mesonephric epithelium development and biological process this GO :1902533 and positive regulation of intracellular signal transduction and biological process, this GO :0005104 : fibroblast growth factor receptor binding, this GO :0005104 : fibroblast growth factor receptor binding and also this GO :0005515 : protein binding and also this GO :0008083 : growth factor activity and also this GO :0008201 : heparin binding and also this GO :0030544 : Hsp70 protein binding and also this GO :0044548 : S100 protein binding, this GO :0005515 : protein binding, this GO :0008083 : growth factor activity, this GO :0008201 : heparin binding, this GO :0030544 : Hsp70 protein binding, this GO :0044548 : S100 protein binding, fibroblast growth factor 1 (acidic) |
| Identity: |
3665 |
| Gene: |
FGF1 |
More about : FGF1 |
| Long gene name: |
fibroblast growth factor 1 |
| Synonyms gene: |
FGFA |
| Synonyms gene name: |
fibroblast growth factor 1 (acidic) |
| Synonyms: |
AFGF ECGF ECGFA ECGFB HBGF1 ECGF-beta FGF-alpha GLIO703 |
| Synonyms name: |
alpha endothelial cell growth factor, beta , heparin-binding growth factor 1 endothelial cell growth factor |
| Locus: |
5q31, 3 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
X59065 |
| Entrez gene record: |
2246 |
| Pubmed identfication: |
1697263 3523756 |
| RefSeq identity: |
NM_000800 |
| Classification: |
Endogenous ligands |
| Havana BLAST/BLAT: |
OTTHUMG00000059703 |