| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
PNPT1 |
| Specificity: |
This antibody reacts specifically with PNPT1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Mitochondrion, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human PNPT1 |
| Gene ID: |
87178 |
| Swiss Prot: |
Q8TCS8 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Binds to the RNA subunit of ribonuclease P, Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction, Component of the mitochondrial degradosome (mtEXO) complex, In the cytoplasm, MRP RNA and miR-221 microRNA, Mitochondrial intermembrane factor with RNA-processing exoribonulease activity, Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, Plays a role in mitochondrial morphogenesis and respiration, Plays also a role in RNA cell surveillance by cleaning up oxidized RNAs, Regulates the stability of specific mature miRNAs in melanoma cells, Required for correct processing and polyadenylation of mitochondrial mRNAs, degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, into the mitochondrial matrix, like the 5S RNA, preferentially, regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels, resulting in a growth arrest in melanoma cells, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity, specifically and selectively degrades miR-221, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, RNA-binding protein implicated in numerous RNA metabolic processes |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
PNPase OLD35 old-35 |
| Also known as: |
PNPT1 Polyclonal Antibody |
| Other name: |
Anti-PNPT1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
PNPT1 |
| Short name: |
Anti-PNPT1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-PNPT1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
23166 |
| Gene: |
PNPT1 |
More about : PNPT1 |
| Long gene name: |
polyribonucleotide nucleotidyltransferase 1 |
| Synonyms gene: |
DFNB70 |
| Synonyms gene name: |
autosomal recessive 70 , deafness |
| Synonyms name: |
RNA exonuclease , polynucleotide phosphorylase 3', -5' |
| Locus: |
2p16, 1 |
| Discovery year: |
2003-09-25 |
| GenBank acession: |
BC053660 |
| Entrez gene record: |
87178 |
| Pubmed identfication: |
12419256 |
| RefSeq identity: |
NM_033109 |
| Classification: |
Deafness associated genes |
| Havana BLAST/BLAT: |
OTTHUMG00000129335 |