| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
RNA polymerase II |
| Specificity: |
This antibody reacts specifically with RNA polymerase II |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Pol II/RNA polymerase II |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Additionally, Although Pol II function requires the cooperation of all twelve subunits, The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1, Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, Without proper function of the large subunit, a groove that is necessary for transcription of the DNA template, forms the active center of the Pol II enzyme, mRNA synthesis and subsequent transcription elongation cannot occur, the large subunit participates in forming the DNA-binding domain of Pol II, the largest subunit conveys Pol II catalytic activity and, thereby allowing transcription to begin, together with the second largest subunit, 840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats, RNA polymerase II (Pol II) is an enzyme that is composed of twelve subunits and is responsible for the transcription of protein-coding genes |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
RBP4 |
| Also known as: |
RNA polymerase II Polyclonal Antibody |
| Other name: |
Anti-RNA polymerase II Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
RNA polymerase II |
| Short name: |
Anti-RNA polymerase II (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-RNA polymerizing enzyme II (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
9191 |
| Gene: |
POLR2D |
More about : POLR2D |
| Long gene name: |
RNA polymerase II subunit D |
| Synonyms gene name: |
polymerase (RNA) II (DNA directed) polypeptide D |
| Synonyms name: |
RNA polymerase II subunit hsRBP4 |
| Locus: |
2q14, 3 |
| Discovery year: |
1993-12-07 |
| GenBank acession: |
U85510 |
| Entrez gene record: |
5433 |
| Pubmed identfication: |
9528765 |
| RefSeq identity: |
NM_004805 |
| Classification: |
RNA polymerase subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000131531 |