| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
CENPB |
| Specificity: |
This antibody reacts specifically with CENPB |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human CENPB |
| Gene ID: |
1059 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
(referenced from Entrez Gene), It is a DNA-binding protein containing a helix loop helix DNA binding motif at the N-terminus, It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere , The DNA binding domain recognizes and binds a 17 bp sequence (CENPB box) in the centromeric satellite DNA, This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes, and a dimerization domain at the C-terminus, This gene product is a highly conserved protein associated with the centromere |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
CENPB Polyclonal Antibody |
| Other name: |
Anti-CENPB Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
CENPB |
| Short name: |
Anti-CENPB (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-CENPB (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
1852 |
| Gene: |
CENPB |
More about : CENPB |
| Long gene name: |
centromere protein B |
| Synonyms gene name: |
80kDa , centromere protein B (80kD) centromere protein B |
| Locus: |
20p13 |
| Discovery year: |
1988-05-31 |
| GenBank acession: |
X05299 |
| Entrez gene record: |
1059 |
| Pubmed identfication: |
8406460 11884609 |
| RefSeq identity: |
NM_001810 |
| Classification: |
Helix-turn-helix CENPB type domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000031761 |