| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
15 Lipoxygenase 1 |
| Specificity: |
This antibody reacts specifically with 15 Lipoxygenase 1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human ALOX15/15 Lipoxygenase 1 |
| Gene ID: |
246 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
15-Lipoxygenase (15-LO) acts in physiological membrane remodeling and the pathogenesis of atherosclerosis, IL-4 and IL-13 play important roles in transactivating the 15-LO gene, It is highly regulated and expressed in a tissue- and cell-type-specific fashion, Overexpression of 15-LO type 1 in prostate cancer contributes to the cancer progression by regulating IGF-1R expression and activation, The Lox enzymes in mammals include 12-LO and 15-LO, The metabolism of arachidonic acid leads to the generation of biologically active metabolites that have been implicated in cell growth and proliferation, and carcinogenesis, arachidonic acid, as well as inducing structural and metabolic changes in the cell, as well as survival and apoptosis, inflammation, thus initiating lipoperoxidation of membranes and synthesis of signaling molecules, which are classified with respect to their positional specificity of the deoxygenation of their most common substrate, Lipoxygenases are a family of enzymes which dioxygenate unsaturated fatty acids |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
15 LIPOXYGENASE RETICULOCYTE ARACHIDONATE, 15 LOX 1, 15-LOX, 15LOX 1, ALOX15, Arachidonate 15 lipoxygenase, Arachidonate 15-lipoxygenase, Arachidonate omega 6 lipoxygenase, Arachidonate omega-6 lipoxygenase, LOX15_HUMAN, 15 LOX |
| Also known as: |
15 Lipoxygenase 1 Polyclonal Antibody |
| Other name: |
Anti-15 Lipoxygenase 1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
15 Lipoxygenase 1 |
| Short name: |
Anti-15 Lipoxygenase 1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-15 Lipoxygenase 1 (polyclonal) |
| Alternative technique: |
polyclonals |