| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
HARS |
| Specificity: |
This antibody reacts specifically with HARS |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human HARS |
| Gene ID: |
3035 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Rat (Rattus norvegicus), Mouse (Mus musculus) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Several transcript variantsencoding different isoforms have been found for this gene, The enzyme is responsible forthe synthesis of histidyl-transfer RNA, The gene is located in ahead-to-head orientation with HARSL on chromosome five, The gene productis a frequent target of auto in the human autoimmunedisease polymyositis/dermatomyositis, The protein encoded bythis gene is a cytoplasmic enzyme which belongs to the class IIfamily of aminoacyl-tRNA synthetases, where thehomologous genes share a bidirectional promoter, which is essential for theincorporation of histidine into proteins, Aminoacyl-tRNA synthetases are a class of enzymes thatcharge tRNAs with their cognate amino acids |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
HARS Polyclonal Antibody |
| Other name: |
Anti-HARS Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
HARS |
| Short name: |
Anti-HARS (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-HARS (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
4816 |
| Gene: |
HARS |
More about : HARS |
| Long gene name: |
histidyl-tRNA synthetase |
| Synonyms name: |
cytoplasmic , histidine tRNA ligase 1 |
| Locus: |
5q31, 3 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
AK000498 |
| Entrez gene record: |
3035 |
| RefSeq identity: |
NM_002109 |
| Classification: |
Aminoacyl tRNA synthetases, Class II |
| Havana BLAST/BLAT: |
OTTHUMG00000129502 |