| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
SIK1 |
| Specificity: |
This antibody reacts specifically with SIK1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Snf1lk |
| Gene ID: |
102724428 |
| Swiss Prot: |
P57059 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Acts as a regulator of hepatic gluconeogenesis by phosphorylating and repressing the CREB-specific coactivators CRTC1/TORC1 and CRTC2/TORC2, Acts as a regulator of muscle cells by phosphorylating and inhibiting class II histone deacetylases HDAC4 and HDAC5, Acts as a tumor suppressor and plays a key role in p53/TP53-dependent anoikis, Also regulates hepatic lipogenesis by phosphorylating and inhibiting SREBF1, Also required during cardiomyogenesis by regulating the exit of cardiomyoblasts from the cell cycle via down-regulation of CDKN1C/p57Kip2, CRTC1/TORC1 and CRTC2/TORC2, HDAC5, In concert with CRTC1/TORC1, PPME1, Part of a sodium-sensing signaling network, Phosphorylates HDAC4, SIK1 is activated by CaMK1 and phosphorylates PPME1 subunit of protein phosphatase 2A (PP2A), SREBF1, a type of apoptosis triggered by cell detachment: required for phosphorylation of p53/TP53 in response to loss of adhesion and is able to suppress metastasis, gluconeogenesis and lipogenesis regulation, leading to dephosphorylation of sodium/potassium-transporting ATPase ATP1A1 and subsequent increase activity of ATP1A1, leading to inhibit CREB activity, leading to promote expression of MEF2 target genes in myocytes, muscle growth and differentiation and tumor suppression, probably by mediating phosphorylation of PPME1: following increases in intracellular sodium, regulates the light-induced entrainment of the circadian clock by attenuating PER1 induction, represses CREB-mediated transcription of PER1 by phosphorylating and deactivating CRTC1/TORC1 (By similarity), Serine/threonine-protein kinase involved in various processes such as cell cycle regulation |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
msk |
| Also known as: |
SIK1 Polyclonal Antibody |
| Other name: |
Anti-SIK1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
SIK1 |
| Short name: |
Anti-SIK1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-salt-inducible kinase 1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
SIK1 and IDBG-4715 and ENSG00000142178 and 150094, SIK1 and IDBG-639805 and ENSBTAG00000011437 and 100337254, Sik1 and IDBG-164811 and ENSMUSG00000024042 and 17691, nuclei, this GO :0000287 and magnesium ion binding and molecular function this GO :0002028 and regulation of sodium ion transport and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0007049 and cell cycle and biological process this GO :0007346 and regulation of mitotic cell cycle and biological process this GO :0008140 and cAMP response element binding protein binding and molecular function this GO :0010830 and regulation of myotube differentiation and biological process this GO :0010868 and negative regulation of triglyceride biosynthetic process and biological process this GO :0016772 and transferase activity, this GO :0000287 : magnesium ion binding, this GO :0000287 : magnesium ion binding and also this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0008140 : cAMP response element binding protein binding and also this GO :0016772 : transferase activity, this GO :0004672 : protein kinase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0008140 : cAMP response element binding protein binding, this GO :0016772 : transferase activity, this GO :0019901 : protein kinase binding, this GO :0071889 : 14-3-3 protein binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0019901 : protein kinase binding and also this GO :0071889 : 14-3-3 protein binding, transferring phosphorus-containing groups and molecular function this GO :0019901 and protein kinase binding and molecular function this GO :0032792 and negative regulation of CREB transcription factor activity and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0045595 and regulation of cell differentiation and biological process this GO :0045721 and negative regulation of gluconeogenesis and biological process this GO :0046777 and protein autophosphorylation and biological process this GO :0055007 and cardiac muscle cell differentiation and biological process this GO :0071889 and 14-3-3 protein binding and molecular function this GO :2000210 and positive regulation of anoikis and biological process, salt-inducible kinase 1 |
| Identity: |
11142 |
| Gene: |
SIK1 |
More about : SIK1 |
| Long gene name: |
salt inducible kinase 1 |
| Synonyms gene: |
SNF1LK |
| Synonyms gene name: |
SNF1-like kinase |
| Synonyms name: |
myocardial SNF1-like kinase |
| Locus: |
21q22, 3 |
| Discovery year: |
2000-05-23 |
| GenBank acession: |
BC038504 |
| Entrez gene record: |
150094 |
| Pubmed identfication: |
7893599 |
| RefSeq identity: |
NM_173354 |
| Havana BLAST/BLAT: |
OTTHUMG00000086874 |