| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
ID3 |
| Specificity: |
This antibody reacts specifically with ID3 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human ID3 |
| Gene ID: |
3399 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer, May inhibit other transcription factors, thereby inhibiting DNA binding, ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
HEIR-1 bHLHb25 |
| Also known as: |
ID3 Polyclonal Antibody |
| Other name: |
Anti-ID3 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
ID3 |
| Short name: |
Anti-ID3 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, dominant negative helix-loop-helix protein (polyclonal), antibody to-inhibitor on Desoxyribonucleic acid binding 3 |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
DNA-templated and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0007417 and central nervous system development and biological process this GO :0007507 and heart development and biological process this GO :0007517 and muscle organ development and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0009611 and response to wounding and biological process this GO :0015630 and microtubule cytoskeleton and cellular component this GO :0019904 and protein domain specific binding and molecular function this GO :0030182 and neuron differentiation and biological process this GO :0030855 and epithelial cell differentiation and biological process this GO :0030903 and notochord development and biological process this GO :0042476 and odontogenesis and biological process this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043433 and negative regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0045662 and negative regulation of myoblast differentiation and biological process this GO :0045668 and negative regulation of osteoblast differentiation and biological process this GO :0045892 and negative regulation of transcription, DNA-templated and biological process this GO :0046983 and protein dimerization activity and molecular function this GO :0051726 and regulation of cell cycle and biological process, ID3 and IDBG-645454 and ENSBTAG00000030425 and 538690, ID3 and IDBG-93963 and ENSG00000117318 and 3399, Id3 and IDBG-197682 and ENSMUSG00000007872 and 15903, bHLHb25 and HEIR-1, dominant negative helix-loop-helix protein, nuclei, protein dimerization activity, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0001656 and metanephros development and biological process this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0003714 and transcription corepressor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006275 and regulation of DNA replication and biological process this GO :0006351 and transcription, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0003714 : transcription corepressor activity and also this GO :0005515 : protein binding and also this GO :0008134 : transcription factor binding and also this GO :0019904 : protein domain specific binding and also this GO :0046983 : protein dimerization activity, this GO :0003714 : transcription corepressor activity, this GO :0005515 : protein binding, this GO :0008134 : transcription factor binding, this GO :0019904 : protein domain specific binding, this GO :0046983 : protein dimerization activity, inhibitor of DNA binding 3 |
| Identity: |
5362 |
| Gene: |
ID3 |
More about : ID3 |
| Long gene name: |
HLH protein , inhibitor of DNA binding 3 |
| Synonyms gene name: |
dominant negative helix-loop-helix protein , inhibitor of DNA binding 3 |
| Synonyms name: |
inhibitor of differentiation 3 |
| Locus: |
1p36, 12 |
| Discovery year: |
1994-12-19 |
| GenBank acession: |
X69111 |
| Entrez gene record: |
3399 |
| Pubmed identfication: |
1628620 |
| RefSeq identity: |
NM_002167 |
| Classification: |
Basic helix-loop-helix proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000003229 |