| Antigen detectable with this ELISA kit: |
Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
062ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1), The concentration of Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1) |
| Short name: |
Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
complementation family 1 (includes overlapping antisense sequence)) Enzyme-linked immunosorbent assay reagent, Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (excision repair cross-complementing rodent repair deficiency |
| Alternative technique: |
elisas |
| Alternative to gene target: |
3'-to lesion and biological process this GO :0006296 and nucleotide-excision repair, 5'-to lesion and biological process this GO :0006302 and double-strand break repair and biological process this GO :0006310 and DNA recombination and biological process this GO :0006312 and mitotic recombination and biological process this GO :0006949 and syncytium formation and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0006979 and response to oxidative stress and biological process this GO :0007281 and germ cell development and biological process this GO :0007283 and spermatogenesis and biological process this GO :0007584 and response to nutrient and biological process this GO :0008022 and protein C-terminus binding and molecular function this GO :0008283 and cell proliferation and biological process this GO :0008584 and male this GO nad development and biological process this GO :0009650 and UV protection and biological process this GO :0009744 and response to sucrose stimulus and biological process this GO :0010165 and response to X-ray and biological process this GO :0010259 and multicellular organismal aging and biological process this GO :0017025 and TBP-class protein binding and molecular function this GO :0019904 and protein domain specific binding and molecular function this GO :0032205 and negative regulation of telomere maintenance and biological process this GO :0035166 and post-embryonic hemopoiesis and biological process this GO :0035264 and multicellular organism growth and biological process this GO :0036297 and interstrand cross-link repair and biological process this GO :0043566 and structure-specific DNA binding and molecular function this GO :0045190 and isotype switching and biological process this GO :0048468 and cell development and biological process this GO :0048477 and oogenesis and biological process this GO :0048568 and embryonic organ development and biological process this GO :0051276 and chromosome organization and biological process this GO :0090305 and nucleic acid phosphodiester bond hydrolysis and biological process, COFS4 and RAD10 and UV20, DNA damage removal and biological process this GO :0000720 and pyrimidine dimer repair by nucleotide-excision repair and biological process this GO :0000737 and DNA catabolic process, DNA incision, DNA incision, ERCC1 and IDBG-57509 and ENSG00000012061 and 2067, ERCC1 and IDBG-639466 and ENSBTAG00000017355 and 615637, Ercc1 and IDBG-150201 and ENSMUSG00000003549 and 13870, complementation group 1 (includes overlapping antisense sequence), endonucleolytic and biological process this GO :0000784 and nuclear chromosome, nuclei, structure-specific DNA binding, telomeric region and cellular component this GO :0001094 and TFIID-class transcription factor binding and molecular function this GO :0001302 and replicative cell aging and biological process this GO :0003677 and DNA binding and molecular function this GO :0003684 and damaged DNA binding and molecular function this GO :0003697 and single-stranded DNA binding and molecular function this GO :0004519 and endonuclease activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005669 and transcription factor TFIID complex and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006281 and DNA repair and biological process this GO :0006283 and transcription-coupled nucleotide-excision repair and biological process this GO :0006289 and nucleotide-excision repair and biological process this GO :0006295 and nucleotide-excision repair, this GO :0000014 and single-stranded DNA endodeoxyribonuclease activity and molecular function this GO :0000109 and nucleotide-excision repair complex and cellular component this GO :0000718 and nucleotide-excision repair, this GO :0000014 : single-stranded DNA endodeoxyribonuclease activity, this GO :0000014 : single-stranded DNA endodeoxyribonuclease activity and also this GO :0001094 : TFIID-class transcription factor binding and also this GO :0003677 : DNA binding and also this GO :0003684 : damaged DNA binding and also this GO :0003697 : single-stranded DNA binding and also this GO :0004519 : endonuclease activity and also this GO :0005515 : protein binding and also this GO :0008022 : protein C-terminus binding and also this GO :0017025 : TBP-class protein binding and also this GO :0019904 : protein domain specific binding and also this GO :0043566 : structure-specific DNA binding, this GO :0001094 : TFIID-class transcription factor binding, this GO :0003677 : DNA binding, this GO :0003684 : damaged DNA binding, this GO :0003697 : single-stranded DNA binding, this GO :0004519 : endonuclease activity, this GO :0005515 : protein binding, this GO :0008022 : protein C-terminus binding, this GO :0017025 : TBP-class protein binding, this GO :0019904 : protein domain specific binding, this GO :0043566 : structure-specific DNA binding, excision repair cross-complementing rodent repair deficiency |
| Identity: |
3433 |
| Gene: |
ERCC1 |
More about : ERCC1 |
| Long gene name: |
ERCC excision repair 1, endonuclease non-catalytic subunit |
| Synonyms gene name: |
complementation group 1 (includes overlapping antisense sequence) excision repair cross-complementation group 1 , excision repair cross-complementing rodent repair deficiency |
| Synonyms: |
RAD10 |
| Locus: |
19q13, 32 |
| Discovery year: |
2001-06-22 |
| Entrez gene record: |
2067 |
| Pubmed identfication: |
6462228 26939044 |
| RefSeq identity: |
NM_001983 |
| Classification: |
ERCC excision repair associated |
| Havana BLAST/BLAT: |
OTTHUMG00000182121 |