| Antigen detectable with this ELISA kit: |
Epithelial Cell Adhesion Molecule (EPCAM) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
1000pg/mL, 125pg/mL, 250pg/mL, 31, 500pg/mL, 62, 25-2000pg/mL The standard curve concentrations used for the ELISA&rsquo, 25pg/mL, 31, 5pg/mL, s were 2000pg/mL |
| Sensitivity: |
9pg/mL, The minimum detectable dose of this kit is typically less than 13 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Epithelial Cell Adhesion Molecule (EPCAM), The concentration of Epithelial Cell Adhesion Molecule (EPCAM) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epithelial Cell Adhesion Molecule (EPCAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Epithelial Cell Adhesion Molecule (EPCAM), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Description: |
present in lysates used as reference for ELISA quantification of these molecules and their subunits, Whole adhesion and interacting molecules are |
| Gene target: |
Epithelial Adhesion Molecule (EPCAM) |
| Short name: |
Epithelial Cell Adhesion Molecule (EPCAM) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Epithelial cellular Adhesion Molecule (epithelial cellular adhesion molecule) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
Cell surfaces, DIAR5 and EGP-2 and EGP314 and EGP40 and ESA and HNPCC8 and KS1/4 and KSA and M4S1 and MIC18 and MK-1 and TACSTD1 and TROP1, EPCAM and IDBG-50831 and ENSG00000119888 and 4072, EPCAM and IDBG-633617 and ENSBTAG00000006474 and 514039, Epcam and IDBG-201437 and ENSMUSG00000045394 and 17075, protein complex binding, this GO :0001657 and ureteric bud development and biological process this GO :0005515 and protein binding and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0005923 and tight junction and cellular component this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0009986 and cell surface and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0016323 and basolateral plasma membrane and cellular component this GO :0016324 and apical plasma membrane and cellular component this GO :0016328 and lateral plasma membrane and cellular component this GO :0023019 and signal transduction involved in regulation of gene expression and biological process this GO :0032403 and protein complex binding and molecular function this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0048863 and stem cell differentiation and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :2000048 and negative regulation of cell-cell adhesion mediated by cadherin and biological process this GO :2000648 and positive regulation of stem cell proliferation and biological process, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0032403 : protein complex binding, this GO :0032403 : protein complex binding, epithelial cell adhesion molecule |
| Identity: |
11529 |
| Gene: |
EPCAM |
More about : EPCAM |
| Long gene name: |
epithelial cell adhesion molecule |
| Synonyms gene: |
M4S1 MIC18 TACSTD1 |
| Synonyms gene name: |
antigen identified by monoclonal antibody AUA1 tumor-associated calcium signal transducer 1 |
| Synonyms: |
Ly74 TROP1 GA733-2 EGP34 EGP40 EGP-2 KSA CD326 Ep-CAM HEA125 KS1/4 MK-1 MH99 MOC31 323/A3 17-1A TACST-1 CO-17A ESA |
| Locus: |
2p21 |
| Discovery year: |
1995-10-02 |
| GenBank acession: |
M33011 |
| Entrez gene record: |
4072 |
| Pubmed identfication: |
8382772 11306819 |
| Classification: |
CD molecules |
| Havana BLAST/BLAT: |
OTTHUMG00000128853 |
| Locus Specific Databases: |
Colon cancer gene variant databases LRG_215 |