| Antigen detectable with this ELISA kit: |
Epidermal Growth Factor (EGF) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
125pg/mL, 3, 3, 7, 15, 31, 62, 25pg/mL, 5pg/mL, 625pg/mL, 813pg/mL, 906-250pg/mL The standard curve concentrations used for the ELISA&rsquo, 906pg/mL, s were 250pg/mL |
| Sensitivity: |
5pg/mL, The minimum detectable dose of this kit is typically less than 1 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell culture supernates and other biological fluids, cell lysates, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Epidermal Growth Factor (EGF), The concentration of Epidermal Growth Factor (EGF) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epidermal Growth Factor (EGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Epidermal Growth Factor (EGF), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Epidermal Growth Factor (EGF) |
| Short name: |
Epidermal Growth Factor (EGF) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Epidermal Growth Factor (epidermal growth factor) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
DNA-templated and biological process this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048015 and phosphatidylinositol-mediated signaling and biological process this GO :0048754 and branching morphogenesis of an epithelial tube and biological process this GO :0050730 and regulation of peptidyl-tyrosine phosphorylation and biological process this GO :0051048 and negative regulation of secretion and biological process this GO :0060749 and mammary gland alveolus development and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0070371 and ERK1 and ERK2 cascade and biological process this GO :0090279 and regulation of calcium ion import and biological process this GO :0090370 and negative regulation of cholesterol efflux and biological process this GO :1900127 and positive regulation of hyaluronan biosynthetic process and biological process this GO :2000008 and regulation of protein localization to cell surface and biological process this GO :2000060 and positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process and biological process, EGF and IDBG-34100 and ENSG00000138798 and 1950, Egf and IDBG-193432 and ENSMUSG00000028017 and 13645, Extracellular, HOMG4 and URG, this GO :0000186 and activation of MAPKK activity and biological process this GO :0001525 and angiogenesis and biological process this GO :0002576 and platelet degranulation and biological process this GO :0005154 and epidermal growth factor receptor binding and molecular function this GO :0005509 and calcium ion binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005765 and lysosomal membrane and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006260 and DNA replication and biological process this GO :0007165 and signal transduction and biological process this GO :0007171 and activation of transmembrane receptor protein tyrosine kinase activity and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007262 and STAT protein import into nucleus and biological process this GO :0007596 and blood coagulation and biological process this GO :0008083 and growth factor activity and molecular function this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0010800 and positive regulation of peptidyl-threonine phosphorylation and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0018108 and peptidyl-tyrosine phosphorylation and biological process this GO :0021940 and positive regulation of cerebellar granule cell precursor proliferation and biological process this GO :0030168 and platelet activation and biological process this GO :0030297 and transmembrane receptor protein tyrosine kinase activator activity and molecular function this GO :0031093 and platelet alpha granule lumen and cellular component this GO :0035413 and positive regulation of catenin import into nucleus and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0042059 and negative regulation of epidermal growth factor receptor signaling pathway and biological process this GO :0042327 and positive regulation of phosphorylation and biological process this GO :0043388 and positive regulation of DNA binding and biological process this GO :0043406 and positive regulation of MAP kinase activity and biological process this GO :0045087 and innate immune response and biological process this GO :0045741 and positive regulation of epidermal growth factor-activated receptor activity and biological process this GO :0045840 and positive regulation of mitosis and biological process this GO :0045893 and positive regulation of transcription, this GO :0005154 : epidermal growth factor receptor binding, this GO :0005154 : epidermal growth factor receptor binding and also this GO :0005509 : calcium ion binding and also this GO :0005515 : protein binding and also this GO :0008083 : growth factor activity and also this GO :0030297 : transmembrane receptor protein tyrosine kinase activator activity, this GO :0005509 : calcium ion binding, this GO :0005515 : protein binding, this GO :0008083 : growth factor activity, this GO :0030297 : transmembrane receptor protein tyrosine kinase activator activity, transmembrane receptor protein tyrosine kinase activator activity, epidermal growth factor |
| Identity: |
3229 |
| Gene: |
EGF |
More about : EGF |
| Long gene name: |
epidermal growth factor |
| Synonyms gene name: |
epidermal growth factor (beta-urogastrone) |
| Locus: |
4q25 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
X04571 |
| Entrez gene record: |
1950 |
| Havana BLAST/BLAT: |
OTTHUMG00000132044 |