| Antigen detectable with this ELISA kit: |
Ephrin B1 (EFNB1) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
067ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ephrin B1 (EFNB1), The concentration of Ephrin B1 (EFNB1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ephrin B1 (EFNB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Ephrin B1 (EFNB1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Ephrin B1 (EFNB1) |
| Short name: |
Ephrin B1 (EFNB1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Ephrin B1 (ephrin-B1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CFND and CFNS and EFB1 and EFL3 and Elk-L and EPLG2 and LERK2, EFNB1 and IDBG-636377 and ENSBTAG00000015801 and 534413, EFNB1 and IDBG-74050 and ENSG00000090776 and 1947, Efnb1 and IDBG-161815 and ENSMUSG00000031217 and 13641, ephrin receptor binding, nuclei, this GO :0001755 and neural crest cell migration and biological process this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0007155 and cell adhesion and biological process this GO :0007267 and cell-cell signaling and biological process this GO :0007411 and axon guidance and biological process this GO :0009880 and embryonic pattern specification and biological process this GO :0016020 and membrane and cellular component this GO :0042102 and positive regulation of T cell proliferation and biological process this GO :0045121 and membrane raft and cellular component this GO :0045202 and synapse and cellular component this GO :0046875 and ephrin receptor binding and molecular function this GO :0048013 and ephrin receptor signaling pathway and biological process this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0046875 : ephrin receptor binding, this GO :0046875 : ephrin receptor binding, ephrin-B1 |
| Identity: |
3226 |
| Gene: |
EFNB1 |
More about : EFNB1 |
| Long gene name: |
ephrin B1 |
| Synonyms gene: |
EPLG2 CFNS |
| Synonyms gene name: |
craniofrontonasal syndrome (craniofrontonasal dysplasia) ephrin-B1 |
| Synonyms: |
LERK2 Elk-L |
| Locus: |
Xq13, 1 |
| Discovery year: |
1995-01-17 |
| GenBank acession: |
U09303 |
| Entrez gene record: |
1947 |
| Pubmed identfication: |
7774950 16526919 |
| RefSeq identity: |
NM_004429 |
| Classification: |
Ephrins |
| Havana BLAST/BLAT: |
OTTHUMG00000021751 |
| Locus Specific Databases: |
Mental Retardation database |