| Antigen detectable with this ELISA kit: |
Eosinophil Peroxidase (EPX) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
1000pg/mL, 125pg/mL, 250pg/mL, 31, 500pg/mL, 62, 25-2000pg/mL The standard curve concentrations used for the ELISA&rsquo, 25pg/mL, 31, 5pg/mL, s were 2000pg/mL |
| Sensitivity: |
7pg/mL, The minimum detectable dose of this kit is typically less than 12 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Eosinophil Peroxidase (EPX), The concentration of Eosinophil Peroxidase (EPX) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eosinophil Peroxidase (EPX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Eosinophil Peroxidase (EPX), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Eosinophil (EPX) |
| Short name: |
Eosinophil Peroxidase (EPX) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Eosinophil Peroxidase (eosinophil peroxidase) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
EPO and EPP and EPX-PEN, EPX and IDBG-60745 and ENSG00000121053 and 8288, Epx and IDBG-207787 and ENSMUSG00000052234 and 13861, metal ion binding, multiple, this GO :0002215 and defense response to nematode and biological process this GO :0004601 and peroxidase activity and molecular function this GO :0006979 and response to oxidative stress and biological process this GO :0020037 and heme binding and molecular function this GO :0032693 and negative regulation of interleukin-10 production and biological process this GO :0032714 and negative regulation of interleukin-5 production and biological process this GO :0032753 and positive regulation of interleukin-4 production and biological process this GO :0042744 and hydrogen peroxide catabolic process and biological process this GO :0046872 and metal ion binding and molecular function this GO :0055114 and oxidation-reduction process and biological process this GO :0072677 and eosinophil migration and biological process, this GO :0004601 : peroxidase activity, this GO :0004601 : peroxidase activity and also this GO :0020037 : heme binding and also this GO :0046872 : metal ion binding, this GO :0020037 : heme binding, this GO :0046872 : metal ion binding, eosinophil peroxidase |
| Identity: |
3423 |
| Gene: |
EPX |
More about : EPX |
| Long gene name: |
eosinophil peroxidase |
| Synonyms: |
EPO EPP EPX-PEN |
| Locus: |
17q22 |
| Discovery year: |
1998-11-24 |
| GenBank acession: |
M26515 |
| Entrez gene record: |
8288 |
| Pubmed identfication: |
2550461 2541222 |
| RefSeq identity: |
NM_000502 |
| Havana BLAST/BLAT: |
OTTHUMG00000178802 |