| Antigen detectable with this ELISA kit: |
Endothelin Converting Enzyme 1 (ECE1) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
1000pg/mL, 125pg/mL, 250pg/mL, 31, 500pg/mL, 62, 25-2000pg/mL The standard curve concentrations used for the ELISA&rsquo, 25pg/mL, 31, 5pg/mL, s were 2000pg/mL |
| Sensitivity: |
9pg/mL, The minimum detectable dose of this kit is typically less than 12 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endothelin Converting Enzyme 1 (ECE1), The concentration of Endothelin Converting Enzyme 1 (ECE1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endothelin Converting Enzyme 1 (ECE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Endothelin Converting Enzyme 1 (ECE1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Description: |
Activating enzymes will cut off the domain that is biological active to become functional, If the substrate is DNA they are called restriction enzymes, Enzymes are cleaving the substrate |
| Gene target: |
Endothelin Converting 1 (ECE1) |
| Short name: |
Endothelin Converting Enzyme 1 (ECE1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, enzymes, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Endothelin Converting Enzyme 1 (endothelin converting enzyme 1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
enzymes |
| Alternative to gene target: |
Cell surfaces, ECE, ECE1 and IDBG-645619 and ENSBTAG00000002977 and 281133, ECE1 and IDBG-93094 and ENSG00000117298 and 1889, Ece1 and IDBG-199213 and ENSMUSG00000057530 and 230857, metal ion binding, this GO :0001921 and positive regulation of receptor recycling and biological process this GO :0003100 and regulation of systemic arterial blood pressure by endothelin and biological process this GO :0004175 and endopeptidase activity and molecular function this GO :0004222 and metalloendopeptidase activity and molecular function this GO :0005765 and lysosomal membrane and cellular component this GO :0005768 and endosome and cellular component this GO :0005769 and early endosome and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006508 and proteolysis and biological process this GO :0007507 and heart development and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0010814 and substance P catabolic process and biological process this GO :0010815 and bradykinin catabolic process and biological process this GO :0010816 and calcitonin catabolic process and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0016485 and protein processing and biological process this GO :0016486 and peptide hormone processing and biological process this GO :0017046 and peptide hormone binding and molecular function this GO :0019229 and regulation of vasoconstriction and biological process this GO :0031302 and intrinsic to endosome membrane and cellular component this GO :0031982 and vesicle and cellular component this GO :0033093 and Weibel-Palade body and cellular component this GO :0034959 and endothelin maturation and biological process this GO :0042447 and hormone catabolic process and biological process this GO :0042733 and embryonic digit morphogenesis and biological process this GO :0042803 and protein homodimerization activity and molecular function this GO :0043583 and ear development and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0060037 and pharyngeal system development and biological process this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0004175 : endopeptidase activity, this GO :0004175 : endopeptidase activity and also this GO :0004222 : metalloendopeptidase activity and also this GO :0017046 : peptide hormone binding and also this GO :0042803 : protein homodimerization activity and also this GO :0046872 : metal ion binding, this GO :0004222 : metalloendopeptidase activity, this GO :0017046 : peptide hormone binding, this GO :0042803 : protein homodimerization activity, this GO :0046872 : metal ion binding, endothelin converting enzyme 1 |
| Identity: |
3146 |
| Gene: |
ECE1 |
More about : ECE1 |
| Long gene name: |
endothelin converting enzyme 1 |
| Synonyms gene: |
ECE |
| Locus: |
1p36, 12 |
| Discovery year: |
1995-12-08 |
| GenBank acession: |
D49471 |
| Entrez gene record: |
1889 |
| Pubmed identfication: |
7805846 7864876 17592116 |
| RefSeq identity: |
NM_001397 |
| Havana BLAST/BLAT: |
OTTHUMG00000002625 |