| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
cdc25A Ser124 |
| Specificity: |
This antibody reacts specifically with cdc25A (Ser124) |
| Modification: |
Phosphorylation |
| Modification site: |
Ser124 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human cdc25A around the phosphorylation site of Ser124 [SH(p-S)DS] |
| Gene ID: |
993 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
CDC25B, CDC25C is predominantly expressed in G2, The activity of cyclin dependent kinases is also regulated by the phosphorylation status, These three genes function at different phases of the cell cycle, Three CDC25 genes are present in human cells: CDC25A, Whereas CDC25A and CDC25B are expressed throughout the cell cycle, and CDC25C, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases, with peak expression in G1 for CDC25A and in both G1 S phase and G2 for CDC25B, Cyclin dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
CDC25A2, Cell division cycle 25 homolog A S, Cell division cycle 25A, Cell division cycle 25A isoform a, Cell division cycle 25A isoform bv D9Ertd393ev Dual specicity phosphatase CDC25A, EC 3, M phase inducer phosphatase 1, MGC115549, MPIP1_HUMAN, pombe, 1, 3, 48, Cdc 25a |
| Also known as: |
cdc25A (Ser124) Polyclonal Antibody |
| Other name: |
Anti-cdc25A (Ser124) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
cdc25A (Ser124) |
| Short name: |
Anti-cdc25A (Ser124) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-cdc25A (Ser124) (polyclonal) |
| Alternative technique: |
polyclonals |