| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Nucleophosmin |
| Specificity: |
This antibody reacts specifically with Nucleophosmin |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Nucleophosmin |
| Gene ID: |
4869 |
| Swiss Prot: |
P06748 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Acts as a chaperonin for the core histones H3, Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids, Binds ribosome presumably to drive ribosome nuclear export, H2B and H4, In concert with BRCA2, May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules, Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation, Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication, Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA, and regulation of tumor suppressors p53/TP53 and ARF, cell proliferation, centrosome duplication, histone assembly, protein chaperoning, regulates centrosome duplication, Involved in diverse cellular processes such as ribosome biogenesis |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
Nucleophosmin Polyclonal Antibody |
| Other name: |
Anti-Nucleophosmin Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Nucleophosmin |
| Short name: |
Anti-Nucleophosmin (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Nucleophosmin (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
7915 |
| Gene: |
NPM1P13 |
More about : NPM1P13 |
| Long gene name: |
nucleophosmin 1 pseudogene 13 |
| Synonyms gene name: |
nucleophosmin 1 (nucleolar phosphoprotein B23, numatrin) pseudogene 13 |
| Locus: |
7p21, 1 |
| Discovery year: |
1999-07-22 |
| GenBank acession: |
AC004994 |
| Entrez gene record: |
10834 |
| Havana BLAST/BLAT: |
OTTHUMG00000152542 |