| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
ROCK1 Thr455 + Ser456 |
| Specificity: |
This antibody reacts specifically with ROCK1(Thr455/Ser456) |
| Modification: |
Phosphorylation |
| Modification site: |
Thr455/Ser456 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human ROCK1 around the phosphorylation site of Thr455/Ser456 |
| Gene ID: |
6093 |
| Swiss Prot: |
Q13464 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation, Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability, GFAP, Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, Involved in regulation of smooth muscle contraction, LIMK1, LIMK2, MYL9/MLC2, May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles, PFN1 and PPP1R12A, Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing, Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress, Plays a role in terminal erythroid differentiation, Promotes keratinocyte terminal differentiation, Required for centrosome positioning and centrosome-dependent exit from mitosis, actin cytoskeleton organization, cell adhesion and motility via phosphorylation of DAPK3, essential for osteoblast mineralization, neurite retraction, stress fiber and focal adhesion formation, Protein kinase which is a key regulator of actin cytoskeleton and cell polarity |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
p160ROCK |
| Also known as: |
ROCK1(Thr455/Ser456) Polyclonal Antibody |
| Other name: |
Anti-ROCK1(Thr455/Ser456) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
ROCK1(Thr455/Ser456) |
| Short name: |
Anti-ROCK1(Thr455/Ser456) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, coiled-coil containing protein kinase 1(Thr455/Ser456) (polyclonal), antibody to-Rho-associated |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
P160ROCK and ROCK-I, Plasma membranes, ROCK1 and IDBG-1371 and ENSG00000067900 and 6093, ROCK1 and IDBG-644080 and ENSBTAG00000008403 and 785911, Rock1 and IDBG-128359 and ENSMUSG00000024290 and 19877, coiled-coil containing protein kinase 1, this GO :0000139 and this GO lgi membrane and cellular component this GO :0001726 and ruffle and cellular component this GO :0003383 and apical constriction and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005814 and centriole and cellular component this GO :0005829 and cytosol and cellular component this GO :0005856 and cytoskeleton and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006915 and apoptotic process and biological process this GO :0006921 and cellular component disassembly involved in execution phase of apoptosis and biological process this GO :0006939 and smooth muscle contraction and biological process this GO :0007159 and leukocyte cell-cell adhesion and biological process this GO :0007165 and signal transduction and biological process this GO :0007266 and Rho protein signal transduction and biological process this GO :0007411 and axon guidance and biological process this GO :0016525 and negative regulation of angiogenesis and biological process this GO :0016772 and transferase activity, this GO :0004672 : protein kinase activity, this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, this GO :0017048 : Rho GTPase binding, this GO :0046872 : metal ion binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0017048 : Rho GTPase binding and also this GO :0046872 : metal ion binding, transferring phosphorus-containing groups and molecular function this GO :0017048 and Rho GTPase binding and molecular function this GO :0022614 and membrane to membrane docking and biological process this GO :0030027 and lamellipodium and cellular component this GO :0030036 and actin cytoskeleton organization and biological process this GO :0030155 and regulation of cell adhesion and biological process this GO :0032059 and bleb and cellular component this GO :0032060 and bleb assembly and biological process this GO :0032956 and regulation of actin cytoskeleton organization and biological process this GO :0032970 and regulation of actin filament-based process and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0045616 and regulation of keratinocyte differentiation and biological process this GO :0046872 and metal ion binding and molecular function this GO :0050900 and leukocyte migration and biological process this GO :0050901 and leukocyte tethering or rolling and biological process this GO :0051451 and myoblast migration and biological process this GO :0051492 and regulation of stress fiber assembly and biological process this GO :0051893 and regulation of focal adhesion assembly and biological process this GO :0051894 and positive regulation of focal adhesion assembly and biological process this GO :2000114 and regulation of establishment of cell polarity and biological process this GO :2000145 and regulation of cell motility and biological process, Rho-associated |
| Identity: |
10251 |
| Gene: |
ROCK1 |
More about : ROCK1 |
| Long gene name: |
Rho associated coiled-coil containing protein kinase 1 |
| Locus: |
18q11, 1 |
| Discovery year: |
1997-10-10 |
| Entrez gene record: |
6093 |
| Pubmed identfication: |
8617235 |
| RefSeq identity: |
NM_005406 |
| Classification: |
Pleckstrin homology domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000131723 |