| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
BARD1 |
| Specificity: |
This antibody reacts specifically with BARD1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human BARD1 |
| Gene ID: |
580 |
| Swiss Prot: |
Q99728 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function, Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage, Plays a central role in the control of the cell cycle in response to DNA damage, The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability, Probable E3 ubiquitin-protein ligase |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
BARD1 Polyclonal Antibody |
| Other name: |
Anti-BARD1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
BARD1 |
| Short name: |
Anti-BARD1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, early onset associated RING domain 1 (polyclonal), antibody to-breast cancer 1 |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
BARD1 and IDBG-643630 and ENSBTAG00000033690 and 530961, BARD1 and IDBG-80113 and ENSG00000138376 and 580, Bard1 and IDBG-164443 and ENSMUSG00000026196 and 12021, nuclei, protein heterodimerization activity, this GO :0000151 and ubiquitin ligase complex and cellular component this GO :0001894 and tissue homeostasis and biological process this GO :0003723 and RNA binding and molecular function this GO :0004842 and ubiquitin-protein transferase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005730 and nucleolus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006281 and DNA repair and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0007050 and cell cycle arrest and biological process this GO :0008270 and zinc ion binding and molecular function this GO :0016567 and protein ubiquitination and biological process this GO :0016874 and ligase activity and molecular function this GO :0019900 and kinase binding and molecular function this GO :0031436 and BRCA1-BARD1 complex and cellular component this GO :0031441 and negative regulation of mRNA 3'-end processing and biological process this GO :0042325 and regulation of phosphorylation and biological process this GO :0042803 and protein homodimerization activity and molecular function this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043231 and intracellular membrane-bounded organelle and cellular component this GO :0045732 and positive regulation of protein catabolic process and biological process this GO :0046826 and negative regulation of protein export from nucleus and biological process this GO :0046982 and protein heterodimerization activity and molecular function this GO :0070531 and BRCA1-A complex and cellular component this GO :0085020 and protein K6-linked ubiquitination and biological process, this GO :0003723 : RNA binding, this GO :0003723 : RNA binding and also this GO :0004842 : ubiquitin-protein transferase activity and also this GO :0005515 : protein binding and also this GO :0008270 : zinc ion binding and also this GO :0016874 : ligase activity and also this GO :0019900 : kinase binding and also this GO :0042803 : protein homodimerization activity and also this GO :0046982 : protein heterodimerization activity, this GO :0004842 : ubiquitin-protein transferase activity, this GO :0005515 : protein binding, this GO :0008270 : zinc ion binding, this GO :0016874 : ligase activity, this GO :0019900 : kinase binding, this GO :0042803 : protein homodimerization activity, this GO :0046982 : protein heterodimerization activity, BRCA1 associated RING domain 1 |
| Identity: |
952 |
| Gene: |
BARD1 |
More about : BARD1 |
| Long gene name: |
BRCA1 associated RING domain 1 |
| Locus: |
2q35 |
| Discovery year: |
1998-08-05 |
| Entrez gene record: |
580 |
| Pubmed identfication: |
8944023 9425226 15159397 |
| RefSeq identity: |
NM_000465 |
| Classification: |
Ankyrin repeat domain containing BRCA1 B complex |
| Havana BLAST/BLAT: |
OTTHUMG00000133016 |