| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
NCK2 |
| Specificity: |
This antibody reacts specifically with NCK2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human NCK2 |
| Gene ID: |
8440 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Alternate transcriptional splice variants, It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization, The protein contains three SH3 domains and one SH2 domain, The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases, [provided by RefSeq], encoding different isoforms, have been characterized, This gene encodes a member of the NCK family of adaptor proteins |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
NCKbeta |
| Also known as: |
NCK2 Polyclonal Antibody |
| Other name: |
Anti- NCK2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
NCK2 |
| Short name: |
Anti- NCK2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, Antibody to NCK adaptor protein 2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
Cytoplasm, GRB4 and NCKbeta, NCK2 and IDBG-634552 and ENSBTAG00000015205 and 526430, NCK2 and IDBG-64634 and ENSG00000071051 and 8440, Nck2 and IDBG-149650 and ENSMUSG00000066877 and 17974, receptor signaling complex scaffold activity, this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005829 and cytosol and cellular component this GO :0006417 and regulation of translation and biological process this GO :0007015 and actin filament organization and biological process this GO :0007165 and signal transduction and biological process this GO :0007172 and signal complex assembly and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007176 and regulation of epidermal growth factor-activated receptor activity and biological process this GO :0007411 and axon guidance and biological process this GO :0008093 and cytoskeletal adaptor activity and molecular function this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0012506 and vesicle membrane and cellular component this GO :0016477 and cell migration and biological process this GO :0030032 and lamellipodium assembly and biological process this GO :0030159 and receptor signaling complex scaffold activity and molecular function this GO :0030838 and positive regulation of actin filament polymerization and biological process this GO :0042102 and positive regulation of T cell proliferation and biological process this GO :0042110 and T cell activation and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0008093 : cytoskeletal adaptor activity and also this GO :0030159 : receptor signaling complex scaffold activity, this GO :0008093 : cytoskeletal adaptor activity, this GO :0030159 : receptor signaling complex scaffold activity, NCK adaptor protein 2 |
| Identity: |
7665 |
| Gene: |
NCK2 |
More about : NCK2 |
| Long gene name: |
NCK adaptor protein 2 |
| Locus: |
2q12, 2 |
| Discovery year: |
1998-10-29 |
| GenBank acession: |
AF043119 |
| Entrez gene record: |
8440 |
| Pubmed identfication: |
9737977 16752908 |
| RefSeq identity: |
NM_003581 |
| Classification: |
SH2 domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000153116 |