| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Ube2B |
| Specificity: |
This antibody reacts specifically with Ube2B |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Ube2B |
| Gene ID: |
7320 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164', H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), In vitro catalyzes 'Lys-11'-, May be involved in neurite outgrowth, Required for postreplication repair of UV-damaged DNA, and is also a prerequisite for H3K4me and H3K79me formation, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination, elongation by RNA polymerase II, it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1, telomeric silencing, Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
UBC2 HHR6B RAD6B |
| Also known as: |
Ube2B Polyclonal Antibody |
| Other name: |
Anti-Ube2B Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Ube2B |
| Short name: |
Anti-Ube2B (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-ubiquitin-conjugating enzyme E2B (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
12473 |
| Gene: |
UBE2B |
More about : UBE2B |
| Long gene name: |
ubiquitin conjugating enzyme E2 B |
| Synonyms gene name: |
ubiquitin-conjugating enzyme E2B (RAD6 homolog) ubiquitin-conjugating enzyme E2B |
| Locus: |
5q31, 1 |
| Discovery year: |
1992-12-02 |
| GenBank acession: |
M74525 |
| Entrez gene record: |
7320 |
| Pubmed identfication: |
1559696 |
| RefSeq identity: |
NM_003337 |
| Classification: |
Ubiquitin conjugating enzymes E2 |
| Havana BLAST/BLAT: |
OTTHUMG00000129120 |