| Antigen detectable with this ELISA kit: |
DNA Damage Inducible Transcript 3 (DDIT3) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
058ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DNA Damage Inducible Transcript 3 (DDIT3), The concentration of DNA Damage Inducible Transcript 3 (DDIT3) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNA Damage Inducible Transcript 3 (DDIT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain DNA Damage Inducible Transcript 3 (DDIT3), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
DNA Damage Transcript 3 (DDIT3) |
| Short name: |
DNA Damage Transcript 3 (DDIT3) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Desoxyribonucleic acid Damage Inducible Transcript 3 (Desoxyribonucleic acid-damage-inducible transcript 3) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CEBPZ and CHOP and CHOP-10 and CHOP10 and GADD153, DDIT3 and IDBG-42805 and ENSG00000175197 and 1649, DDIT3 and IDBG-635159 and ENSBTAG00000031544 and 777788, DNA-templated and biological process this GO :0006915 and apoptotic process and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0006983 and ER overload response and biological process this GO :0006986 and response to unfolded protein and biological process this GO :0006987 and activation of signaling protein activity involved in unfolded protein response and biological process this GO :0007050 and cell cycle arrest and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0016055 and Wnt signaling pathway and biological process this GO :0030968 and endoplasmic reticulum unfolded protein response and biological process this GO :0032757 and positive regulation of interleukin-8 production and biological process this GO :0034976 and response to endoplasmic reticulum stress and biological process this GO :0042594 and response to starvation and biological process this GO :0042789 and mRNA transcription from RNA polymerase II promoter and biological process this GO :0043161 and proteasome-mediated ubiquitin-dependent protein catabolic process and biological process this GO :0043433 and negative regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0043525 and positive regulation of neuron apoptotic process and biological process this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0043620 and regulation of DNA-dependent transcription in response to stress and biological process this GO :0044267 and cellular protein metabolic process and biological process this GO :0044324 and regulation of transcription involved in anterior/posterior axis specification and biological process this GO :0045087 and innate immune response and biological process this GO :0045454 and cell redox homeostasis and biological process this GO :0045662 and negative regulation of myoblast differentiation and biological process this GO :0045892 and negative regulation of transcription, DNA-templated and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0051209 and release of sequestered calcium ion into cytosol and biological process this GO :0070059 and intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress and biological process this GO :0090090 and negative regulation of canonical Wnt signaling pathway and biological process this GO :2000016 and negative regulation of determination of dorsal identity and biological process, Ddit3 and IDBG-194880 and ENSMUSG00000025408 and 13198, nuclei, sequence-specific DNA binding, this GO :0001955 and blood vessel maturation and biological process this GO :0003677 and DNA binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0003714 and transcription corepressor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005770 and late endosome and cellular component this GO :0006355 and regulation of transcription, this GO :0003677 : DNA binding, this GO :0003677 : DNA binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0003714 : transcription corepressor activity and also this GO :0005515 : protein binding and also this GO :0008134 : transcription factor binding and also this GO :0043565 : sequence-specific DNA binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0003714 : transcription corepressor activity, this GO :0005515 : protein binding, this GO :0008134 : transcription factor binding, this GO :0043565 : sequence-specific DNA binding, DNA-damage-inducible transcript 3 |
| Identity: |
2726 |
| Gene: |
DDIT3 |
More about : DDIT3 |
| Long gene name: |
DNA damage inducible transcript 3 |
| Synonyms gene name: |
DNA-damage-inducible transcript 3 |
| Synonyms: |
CHOP10 GADD153 CHOP |
| Synonyms name: |
C/EBP zeta |
| Locus: |
12q13, 3 |
| Discovery year: |
1991-09-11 |
| GenBank acession: |
BC003637 |
| Entrez gene record: |
1649 |
| Pubmed identfication: |
1990262 |
| RefSeq identity: |
NM_004083 |
| Havana BLAST/BLAT: |
OTTHUMG00000170046 |