| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
HLA-DPB1 |
| Specificity: |
This antibody reacts specifically with HLA-DPB1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Extracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human DPB1 |
| Gene ID: |
3115 |
| Swiss Prot: |
P04440 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, Autophagy is also a source of endogenous peptides, CD74 undergoes a sequential degradation by various proteases, Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound, In addition to APCs, Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, The MHC II molecule bound to a peptide is then transported to the cell membrane surface, The peptide binding cleft accommodates peptides of 10-30 residues, The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released, To produce a MHC class II molecule that presents an antigen, and for this reason this antigen presentation pathway is usually referred to as exogenous, autophagosomes constitutively fuse with MHC class II loading compartments, exogenous antigens must compete with those derived from endogenous components, express MHC class II molecules and CD74 and act as APCs, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide), other cells of the gastrointestinal tract, such as epithelial cells, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer, where they are processed by lysosomal proteases and other hydrolases, which is an unusual trait of the GI tract, Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
HLA-DPB1 Polyclonal Antibody |
| Other name: |
Anti-HLA-DPB1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
HLA-DPB1 |
| Short name: |
Anti-HLA-DPB1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, DP b 1 (polyclonal), class II, antibody to-major histocompatibility aggregate |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
Cell surfaces, DP beta 1, DPB1 and HLA-DP and HLA-DP1B and HLA-DPB, HLA-DPB1 and IDBG-230487 and ENSG00000223865 and 3115, class II, peptide antigen binding, this GO :0000139 and this GO lgi membrane and cellular component this GO :0002504 and antigen processing and presentation of peptide or polysaccharide antigen via MHC class II and biological process this GO :0005515 and protein binding and molecular function this GO :0005765 and lysosomal membrane and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006955 and immune response and biological process this GO :0009986 and cell surface and cellular component this GO :0010008 and endosome membrane and cellular component this GO :0012507 and ER to this GO lgi transport vesicle membrane and cellular component this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0019882 and antigen processing and presentation and biological process this GO :0019886 and antigen processing and presentation of exogenous peptide antigen via MHC class II and biological process this GO :0030658 and transport vesicle membrane and cellular component this GO :0030666 and endocytic vesicle membrane and cellular component this GO :0030669 and clathrin-coated endocytic vesicle membrane and cellular component this GO :0031295 and T cell costimulation and biological process this GO :0032588 and trans- this GO lgi network membrane and cellular component this GO :0032729 and positive regulation of interferon-gamma production and biological process this GO :0042102 and positive regulation of T cell proliferation and biological process this GO :0042605 and peptide antigen binding and molecular function this GO :0042613 and MHC class II protein complex and cellular component this GO :0050852 and T cell receptor signaling pathway and biological process this GO :0050870 and positive regulation of T cell activation and biological process this GO :0060333 and interferon-gamma-mediated signaling pathway and biological process this GO :0071556 and integral component of lumenal side of endoplasmic reticulum membrane and cellular component, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0042605 : peptide antigen binding, this GO :0042605 : peptide antigen binding, major histocompatibility complex |
| Identity: |
4940 |
| Gene: |
HLA-DPB1 |
More about : HLA-DPB1 |
| Long gene name: |
DP beta 1 , class II, major histocompatibility complex |
| Synonyms gene: |
HLA-DP1B |
| Locus: |
6p21, 32 |
| Discovery year: |
2001-06-22 |
| Entrez gene record: |
3115 |
| RefSeq identity: |
NM_002121 |
| Classification: |
C1-set domain containing Histocompatibility complex |
| Havana BLAST/BLAT: |
OTTHUMG00000031076 |
| Locus Specific Databases: |
IMGT/HLA Database |