| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
GOT2 |
| Specificity: |
This antibody reacts specifically with GOT2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human GOT2 |
| Gene ID: |
2806 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, High levels are also found after myocardial infarction, In liver about 80% of the enzyme activity is mitochondrial in origin, Serum GOT [with SGPT] levels are usually elevated in states of hepatocellular injury (injury to the liver cells), The 2 isoenzymes are homodimeric, The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene, the highest levels are associated with hepatitis of a viral origin, when SGPT levels are lower, whereas in serum the enzyme activity is largely cytosolic, GOT2 [Glutamate oxaloacetate transaminase] is a ubiquitous pyridoxal phosphate dependent enzyme which exists in both mitochondrial and cytosolic forms |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
mitAAT KATIV KAT4 KYAT4 |
| Also known as: |
GOT2 Polyclonal Antibody |
| Other name: |
Anti-GOT2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
GOT2 |
| Short name: |
Anti-GOT2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-GOT2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
4433 |
| Gene: |
GOT2 |
More about : GOT2 |
| Long gene name: |
glutamic-oxaloacetic transaminase 2 |
| Synonyms gene name: |
glutamic-oxaloacetic transaminase 2, mitochondrial , mitochondrial (aspartate aminotransferase 2) glutamic-oxaloacetic transaminase 2 |
| Synonyms name: |
kynurenine aminotransferase IV aspartate aminotransferase 2 aspartate transaminase 2 |
| Locus: |
16q21 |
| Discovery year: |
2001-06-22 |
| Entrez gene record: |
2806 |
| Pubmed identfication: |
17442055 |
| Havana BLAST/BLAT: |
OTTHUMG00000133769 |