| Antigen detectable with this ELISA kit: |
Dihydroorotate Dehydrogenase (DHODH) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
056ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dihydroorotate Dehydrogenase (DHODH), The concentration of Dihydroorotate Dehydrogenase (DHODH) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dihydroorotate Dehydrogenase (DHODH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Dihydroorotate Dehydrogenase (DHODH), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Dihydroorotate Dehydrogenase (DHODH) |
| Short name: |
Dihydroorotate Dehydrogenase (DHODH) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Dihydroorotate Dehydrogenase (dihydroorotate dehydrogenase (quinone)) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
DHODH and IDBG-41409 and ENSG00000102967 and 1723, DHODH and IDBG-634716 and ENSBTAG00000046908 and, DHOdehase and POADS and URA1, Dhodh and IDBG-191275 and ENSMUSG00000031730 and 56749, Plasma membranes, this GO :0004152 and dihydroorotate dehydrogenase activity and molecular function this GO :0004158 and dihydroorotate oxidase activity and molecular function this GO :0005739 and mitochondrion and cellular component this GO :0005743 and mitochondrial inner membrane and cellular component this GO :0006206 and pyrimidine nucleobase metabolic process and biological process this GO :0006207 and 'de novo' pyrimidine nucleobase biosynthetic process and biological process this GO :0006222 and UMP biosynthetic process and biological process this GO :0007565 and female pregnancy and biological process this GO :0007595 and lactation and biological process this GO :0008144 and drug binding and molecular function this GO :0010181 and FMN binding and molecular function this GO :0014070 and response to organic cyclic compound and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0031000 and response to caffeine and biological process this GO :0042493 and response to drug and biological process this GO :0042594 and response to starvation and biological process this GO :0043025 and neuronal cell body and cellular component this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0044205 and 'de novo' UMP biosynthetic process and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0046134 and pyrimidine nucleoside biosynthetic process and biological process this GO :0048039 and ubiquinone binding and molecular function this GO :0055086 and nucleobase-containing small molecule metabolic process and biological process this GO :0055114 and oxidation-reduction process and biological process this GO :0090140 and regulation of mitochondrial fission and biological process, this GO :0004152 : dihydroorotate dehydrogenase activity, this GO :0004152 : dihydroorotate dehydrogenase activity and also this GO :0004158 : dihydroorotate oxidase activity and also this GO :0008144 : drug binding and also this GO :0010181 : FMN binding and also this GO :0048039 : ubiquinone binding, this GO :0004158 : dihydroorotate oxidase activity, this GO :0008144 : drug binding, this GO :0010181 : FMN binding, this GO :0048039 : ubiquinone binding, ubiquinone binding, dihydroorotate dehydrogenase (quinone) |
| Identity: |
2867 |
| Gene: |
DHODH |
More about : DHODH |
| Long gene name: |
dihydroorotate dehydrogenase (quinone) |
| Synonyms gene name: |
dihydroorotate dehydrogenase |
| Locus: |
16q22, 2 |
| Discovery year: |
1993-06-29 |
| Entrez gene record: |
1723 |
| Pubmed identfication: |
8211381 |
| RefSeq identity: |
NM_001361 |
| Havana BLAST/BLAT: |
OTTHUMG00000178093 |