| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
GPX4 |
| Specificity: |
This antibody reacts specifically with GPX4 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Glutathione Peroxidase 4 |
| Gene ID: |
2879 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
All mammalian GPx family members, Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation, GPx2, Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, There are several isoforms which differ in their primary structure and localization, also known as gastrointestinal GPx (GI-GPx), can reduce many hydroperoxides including hydroperoxides integrated in membranes, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, first of the GPx family to be discovered, hydroperoxy lipids in low density lipoprotein or thymine, is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract, possess selenocysteine at the active site, Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
PHGPx MCSP |
| Also known as: |
GPX4 Polyclonal Antibody |
| Other name: |
Anti-GPX4 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
GPX4 |
| Short name: |
Anti-GPX4 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-GPX4 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
4556 |
| Gene: |
GPX4 |
More about : GPX4 |
| Long gene name: |
glutathione peroxidase 4 |
| Synonyms gene name: |
glutathione peroxidase 4 (phospholipid hydroperoxidase) |
| Synonyms name: |
phospholipid hydroperoxidase |
| Locus: |
19p13, 3 |
| Discovery year: |
1993-05-03 |
| GenBank acession: |
BC039849 X71973 |
| Entrez gene record: |
2879 |
| Pubmed identfication: |
8287691 8039723 10464096 |
| RefSeq identity: |
NM_002085 |
| Classification: |
Selenoproteins |
| Havana BLAST/BLAT: |
OTTHUMG00000181874 |