| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
ATG18/WIPI1 |
| Specificity: |
This antibody reacts specifically with ATG18/WIPI1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from mouse WIPI1 |
| Gene ID: |
55062 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Consistent with this, Overexpression of WIPI1 disrupted the function of the MPR pathway, Suppression of WIPI1 by interfering RNA indicated that WIPI1 is required for normal endosomal organization and distribution of IGF2R, WIPI1 moved through the same set of endosomal membranes as that followed by mannose-6-phosphate receptor (MPR), WIPI1 was enriched in clathrin-coated vesicles, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids, phosphoinositide interacting 1), such as WIPI1 (WD repeat domain, whereas expression of a double point mutant unable to bind phosphoinositides had no effect, Members of the WIPI subfamily of WD40 repeat proteins |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
FLJ10055 WIPI49 ATG18 ATG18A |
| Also known as: |
ATG18/WIPI1 Polyclonal Antibody |
| Other name: |
Anti-ATG18/WIPI1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
ATG18/WIPI1 |
| Short name: |
Anti-ATG18/WIPI1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-ATG18/WIPI1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
25471 |
| Gene: |
WIPI1 |
More about : WIPI1 |
| Long gene name: |
WD repeat domain, phosphoinositide interacting 1 |
| Locus: |
17q24, 2 |
| Discovery year: |
2005-11-15 |
| Entrez gene record: |
55062 |
| Pubmed identfication: |
15020712 15602573 |
| RefSeq identity: |
NM_017983 |
| Classification: |
Autophagy related WD repeat domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000179873 |