| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
e4G |
| Specificity: |
This antibody reacts specifically with eIF4G |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human eIF4G |
| Gene ID: |
1981 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
A domain at its N-terminus can also interact with the poly(A)-binding protein, Alternative splicing results in multiple transcript variants, The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex, The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex, This complex facilitates the recruitment of mRNA to the ribosome, [provided by RefSeq], some of which are derived from alternative promoter usage, which is a rate-limiting step during the initiation phase of protein synthesis, which may mediate the circularization of mRNA during translation, The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
p220 PARK18 |
| Also known as: |
eIF4G Polyclonal Antibody |
| Other name: |
Anti-eIF4G Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
eIF4G |
| Short name: |
Anti-eIF4G (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-eIF4G (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
3296 |
| Gene: |
EIF4G1 |
More about : EIF4G1 |
| Long gene name: |
eukaryotic translation initiation factor 4 gamma 1 |
| Synonyms gene: |
EIF4G EIF4F |
| Synonyms gene name: |
1 , eukaryotic translation initiation factor 4 gamma |
| Locus: |
3q27, 1 |
| Discovery year: |
1993-06-17 |
| GenBank acession: |
D12686 |
| Entrez gene record: |
1981 |
| Pubmed identfication: |
1429670 9372926 21907011 |
| RefSeq identity: |
NM_182917 |
| Classification: |
Parkinson disease associated genes |
| Havana BLAST/BLAT: |
OTTHUMG00000156784 |