| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
HP1 alpha |
| Specificity: |
This antibody reacts specifically with HP1 alpha |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human HP1 alpha |
| Gene ID: |
23468 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Both domains of HP1 are requiredfor binding to native chromatin in vivo, HP1 alpha may interact with lamin B receptor (LBR), HP1 proteins are relatively small proteins (~25 kDa) with a conserved amino-terminal chromo domain and a structurally related carboxy-terminal motif, It recognizes and binds histone H3 tails methylated at Lys-9, This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane, but they contribute differentially to binding in euchromatin and heterochromatin, leading to epigenetic repression, the chromo shadow domain, HP1 alpha is a component of heterochromatin |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
HP1Hs-alpha HP1 HP1-ALPHA |
| Also known as: |
HP1 alpha Polyclonal Antibody |
| Other name: |
Anti-HP1 alpha Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
HP1 alpha |
| Short name: |
Anti-HP1 alpha (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-HP1 a (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
1555 |
| Gene: |
CBX5 |
More about : CBX5 |
| Long gene name: |
chromobox 5 |
| Synonyms gene name: |
Drosophila) chromobox homolog 5 , chromobox homolog 5 (Drosophila HP1 alpha) chromobox homolog 5 (HP1 alpha homolog |
| Synonyms name: |
HP1 alpha homolog (Drosophila) |
| Locus: |
12q13, 13 |
| Discovery year: |
1999-10-12 |
| GenBank acession: |
U26311 |
| Entrez gene record: |
23468 |
| Pubmed identfication: |
8663349 |
| RefSeq identity: |
NM_012117 |
| Classification: |
Chromobox family |
| Havana BLAST/BLAT: |
OTTHUMG00000169700 |