| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Histone H3 Tri Methyl K27 |
| Specificity: |
This antibody reacts specifically with Histone H3 (Tri Methyl K27) |
| Modification: |
Methylation |
| Modification site: |
Tri Methyl K27 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic methyl peptide derived from human Histone H3 around methylation site of Lys27 |
| Gene ID: |
3020 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
12, 14, 15 and 20, 18 and 23, Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms, H2B, H3 and H4), Histone H3 is primarily acetylated at lysines 9, In most species, Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis, The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, The nucleosome, These modifications occur in response to cell signal stimuli and have a direct effect on gene expression, is the primary building block of chromatin, made up of four core histone proteins (H2A, phosphorylation and methylation, the histone H2B is primarily acetylated at lysines 5, Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
H3 histone family member E pseudogene, H3F3, HIST3H3, Histone H3 3 pseudogene, Histone H3 |
| Also known as: |
Histone H3 (Tri Methyl K27) Antibody |
| Other name: |
Anti-Histone H3 (Tri Methyl K27) |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Description: |
All three forms are highly reactive and rarely observed, CH3, In , It is a very stable group in most molecules, Such hydrocarbon , The anion has eight valence electrons, While the methyl group is usually part of a larger , containing one , it can be found on its own in any of three forms , the group is often , the radical seven and the cation six, , A , Me, abbreviated , alkyl , anion, atom , atoms &mdash, bonded , carbon , cation , derived from , formulas, groups occur in many , hydrogen , is an , methane, methyl group , molecule, or , organic compounds, radical, to three |
| Gene target: |
Histone H3 (Tri Methyl K27) |
| Short name: |
Anti-Histone H3 (Tri Methyl K27) Fluor 594 |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Histone H3 (Tri Methyl K27) |