| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Crkl |
| Specificity: |
This antibody reacts specifically with Crkl |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Intracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Crkl |
| Gene ID: |
1399 |
| Swiss Prot: |
P46109 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Crkl has oncogenic potential, Crkl is a substitute of BCR ABL tyrosine kinase, In addition, Crkl is a 34kDa adaptor protein which has been shown to activate the RAS and JUN kinase signalling pathways and transforms fibroblasts in a RAS dependant manner |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
Crkl Polyclonal Antibody |
| Other name: |
Anti-Crkl Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Crkl |
| Short name: |
Anti-Crkl (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-v-crk sarcoma virus CT10 oncogene homolog (avian)-like (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
2363 |
| Gene: |
CRKL |
More about : CRKL |
| Long gene name: |
CRK like proto-oncogene, adaptor protein |
| Synonyms gene name: |
v-crk avian sarcoma virus CT10 oncogene homolog-like |
| Locus: |
22q11, 21 |
| Discovery year: |
1994-09-14 |
| Entrez gene record: |
1399 |
| Pubmed identfication: |
8361759 8798523 |
| RefSeq identity: |
NM_005207 |
| Classification: |
SH2 domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000150807 |