| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
e4E |
| Specificity: |
This antibody reacts specifically with eIF4E |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human eIF4E |
| Gene ID: |
1977 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Phosphorylation also enhances the interaction of eIF4E with eIF4G, Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA, Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription, eIF4E also influences the overall rate of translation, eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, eIF4E phosphorylation is correlated with increased translational rate in a number of cell types, which form a complex known as eIF4F, eIF4E |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
EIF4E1 |
| Also known as: |
eIF4E Polyclonal Antibody |
| Other name: |
Anti-eIF4E Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
eIF4E |
| Short name: |
Anti-eIF4E (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-eIF4E (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
3287 |
| Gene: |
EIF4E |
More about : EIF4E |
| Long gene name: |
eukaryotic translation initiation factor 4E |
| Synonyms gene: |
EIF4EL1 EIF4F |
| Locus: |
4q23 |
| Discovery year: |
1991-07-09 |
| GenBank acession: |
M15353 |
| Entrez gene record: |
1977 |
| Pubmed identfication: |
9330633 1916814 |
| RefSeq identity: |
NM_001968 |
| Havana BLAST/BLAT: |
OTTHUMG00000161090 |