| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
ZAP70 Tyr315 + Tyr319 |
| Specificity: |
This antibody reacts specifically with ZAP70 (Tyr315 + Tyr319) |
| Modification: |
Phosphorylation |
| Modification site: |
Tyr315 + Tyr319 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human Zap-70 around the phosphorylation site of Tyr315/Tyr319 |
| Gene ID: |
7535 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Jul 2008], Lck and Fyn, Mutations in this gene cause selective T-cell defect, This enzyme, This enzyme is also essential for thymocyte development, Two transcript variants that encode different isoforms have been found for this gene, [provided by RefSeq, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells, and it plays a role in T-cell development and lymphocyte activation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, This gene encodes an enzyme belonging to the protein tyrosine kinase family |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
ZAP-70 STD |
| Also known as: |
ZAP70 (Tyr315 + Tyr319) Antibody |
| Other name: |
Anti-ZAP70 (Tyr315 + Tyr319) |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
ZAP70 (Tyr315 + Tyr319) |
| Short name: |
Anti-ZAP70 (Tyr315 + Tyr319) Fluor 594 |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-zeta-chain (TCR) associated protein kinase 70kDa (Tyr315 and Tyr319) |
| Alternative to gene target: |
Cell surfaces, SRK and STCD and STD and TZK and ZAP-70, ZAP70 and IDBG-62509 and ENSG00000115085 and 7535, ZAP70 and IDBG-629842 and ENSBTAG00000005892 and 504509, Zap70 and IDBG-145089 and ENSMUSG00000026117 and 22637, this GO :0001772 and immunological synapse and cellular component this GO :0001784 and phosphotyrosine binding and molecular function this GO :0002250 and adaptive immune response and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0004715 and non-membrane spanning protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005911 and cell-cell junction and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006955 and immune response and biological process this GO :0016772 and transferase activity, this GO :0001784 : phosphotyrosine binding, this GO :0001784 : phosphotyrosine binding and also this GO :0004672 : protein kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0004715 : non-membrane spanning protein tyrosine kinase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004672 : protein kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0004715 : non-membrane spanning protein tyrosine kinase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups, transferring phosphorus-containing groups and molecular function this GO :0018108 and peptidyl-tyrosine phosphorylation and biological process this GO :0030217 and T cell differentiation and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0042101 and T cell receptor complex and cellular component this GO :0042110 and T cell activation and biological process this GO :0042113 and B cell activation and biological process this GO :0043366 and beta selection and biological process this GO :0045059 and positive thymic T cell selection and biological process this GO :0045060 and negative thymic T cell selection and biological process this GO :0045061 and thymic T cell selection and biological process this GO :0045121 and membrane raft and cellular component this GO :0045582 and positive regulation of T cell differentiation and biological process this GO :0046638 and positive regulation of alpha-beta T cell differentiation and biological process this GO :0046641 and positive regulation of alpha-beta T cell proliferation and biological process this GO :0046777 and protein autophosphorylation and biological process this GO :0050850 and positive regulation of calcium-mediated signaling and biological process this GO :0050852 and T cell receptor signaling pathway and biological process this GO :0070489 and T cell aggregation and biological process this GO :0072678 and T cell migration and biological process, zeta-chain (TCR) associated protein kinase 70kDa |
| Identity: |
12858 |
| Gene: |
ZAP70 |
More about : ZAP70 |
| Long gene name: |
zeta chain of T-cell receptor associated protein kinase 70 |
| Synonyms gene: |
SRK |
| Synonyms gene name: |
zeta-chain (TCR) associated protein kinase (70 kD) zeta-chain (TCR) associated protein kinase 70kDa zeta chain of T cell receptor associated protein kinase 70kDa zeta chain of T cell receptor associated protein kinase 70 |
| Synonyms name: |
tyrosine-protein kinase ZAP-70 |
| Locus: |
2q11, 2 |
| Discovery year: |
1995-05-16 |
| GenBank acession: |
L05148 |
| Entrez gene record: |
7535 |
| Pubmed identfication: |
1423621 |
| RefSeq identity: |
NM_001079 |
| Classification: |
SH2 domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000153060 |
| Locus Specific Databases: |
ZAP70base: Mutation registry for autosomal recessive ZAP70 immunodeficiency LRG_126 |