| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
PKC alpha/beta II Thr638/641 |
| Specificity: |
This antibody reacts specifically with PKC alpha/beta II (Thr638/641) |
| Modification: |
Phosphorylation |
| Modification site: |
Thr638/641 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human PKC alpha/beta II around the phosphorylation site of Thr638/641 |
| Gene ID: |
5578 |
| Swiss Prot: |
P17252 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Dog, Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
PKC alpha is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of a broad range of cellular functions including proliferation, The activation loop threonine (threonine 497 in PKC alpha) of conventional PKCs is phosphorylated by phosphoinositide dependent kinase 1 (PDK1), This phosphorylation is necessary for the autophosphorylation of threonine 638 in the carboxy terminus of PKC alpha, a step that is critical for regulating the rate of PKC alpha dephosphorylation and inactivation, and solute transport, cell cell adhesion, cell extracellular matrix adhesion, cell survival and transcriptional regulation, development, diacylglycerol, differentiation, migration, phosphatidylserine and phorbol esters) of the PKC family of serine/ threonine kinases that are involved in a wide range of physiological processes including mitogenesis, Protein Kinase c alpha (PKC alpha) is an 77 kDa member of the conventional group (cPKCs: sensitive to calcium |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
FLJ25513 DKFZp547J199 IFITMD1 FICCA DSPB3 PKC EKD1 |
| Also known as: |
PKC alpha/beta II (Thr638/641) Antibody |
| Other name: |
Anti-PKC alpha/beta II (Thr638/641) |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
PKC alpha/beta II (Thr638/641) |
| Short name: |
Anti-PKC alpha/beta II (Thr638/641) Fluor 594 |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-PKC a/b II (Thr638/641) |
| Identity: |
30500 |
| Gene: |
PRRT2 |
More about : PRRT2 |
| Long gene name: |
proline rich transmembrane protein 2 |
| Synonyms gene: |
ICCA DYT10 |
| Synonyms gene name: |
infantile convulsions and paroxysmal choreoathetosis dystonia 10 proline-rich transmembrane protein 2 |
| Synonyms name: |
interferon induced transmembrane protein domain containing 1 |
| Locus: |
16p11, 2 |
| Discovery year: |
2005-11-25 |
| GenBank acession: |
BC011405 |
| Entrez gene record: |
112476 |
| Pubmed identfication: |
22101681 22243967 |
| RefSeq identity: |
NM_145239 |
| Classification: |
Proline rich transmembrane proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000177142 |