| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
NPM Thr199 |
| Specificity: |
This antibody reacts specifically with NPM (Thr199) |
| Modification: |
Phosphorylation |
| Modification site: |
Thr199 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human NPM around the phosphorylation site of Thr199 |
| Gene ID: |
4869 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
A number of genes are fusion partners have been characterized, Alternative splicing results in multiple transcript variants, More than a dozen pseudogenes of this gene have been identified, Mutations in this gene are associated with acute myeloid leukemia, Nov 2009], The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway, in particular the anaplastic lymphoma kinase gene on chromosome 2, This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm, [provided by RefSeq |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
B23 NPM |
| Also known as: |
NPM (Thr199) Polyclonal Antibody |
| Other name: |
Anti-NPM (Thr199) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
NPM (Thr199) |
| Short name: |
Anti-NPM (Thr199) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-NPM (Thr199) (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
7910 |
| Gene: |
NPM1 |
More about : NPM1 |
| Long gene name: |
nucleophosmin |
| Synonyms gene name: |
nucleophosmin (nucleolar phosphoprotein B23, numatrin) |
| Synonyms name: |
member 1 , nucleolar phosphoprotein B23 numatrin nucleophosmin/nucleoplasmin family |
| Locus: |
5q35, 1 |
| Discovery year: |
1993-08-24 |
| GenBank acession: |
M26697 |
| Entrez gene record: |
4869 |
| Pubmed identfication: |
8471164 8122112 |
| RefSeq identity: |
NM_002520 |
| Havana BLAST/BLAT: |
OTTHUMG00000130465 |
| Locus Specific Databases: |
LRG_458 |