| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
IRF3 Ser396 |
| Specificity: |
This antibody reacts specifically with IRF3 (Ser396) |
| Modification: |
Phosphorylation |
| Modification site: |
Ser396 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human IRF3 around the phosphorylation site of Ser396 |
| Gene ID: |
3661 |
| Swiss Prot: |
Q14653 |
| Applications: |
IF(ICC), IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(ICC)(1:50-200), IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction, Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages, Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters, This induces a conformational change, a complex which activates the transcription of the type I IFN and ISG genes, double-stranded RNA (dsRNA), is phosphorylated by IKBKE and TBK1 kinases, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), or toll-like receptor (TLR) signaling, Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
IRF3 (Ser396) Polyclonal Antibody |
| Other name: |
Anti-IRF3 (Ser396) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
IRF3 (Ser396) |
| Short name: |
Anti-IRF3 (Ser396) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-interferon regulatory factor 3 (Ser396) (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
DNA-templated and biological process this GO :0006366 and transcription from RNA polymerase II promoter and biological process this GO :0006915 and apoptotic process and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0009617 and response to bacterium and biological process this GO :0016032 and viral process and biological process this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0031663 and lipopolysaccharide-mediated signaling pathway and biological process this GO :0032480 and negative regulation of type I interferon production and biological process this GO :0032481 and positive regulation of type I interferon production and biological process this GO :0032496 and response to lipopolysaccharide and biological process this GO :0032727 and positive regulation of interferon-alpha production and biological process this GO :0032728 and positive regulation of interferon-beta production and biological process this GO :0034138 and toll-like receptor 3 signaling pathway and biological process this GO :0034142 and toll-like receptor 4 signaling pathway and biological process this GO :0035666 and TRIF-dependent toll-like receptor signaling pathway and biological process this GO :0039530 and MDA-5 signaling pathway and biological process this GO :0042802 and identical protein binding and molecular function this GO :0042803 and protein homodimerization activity and molecular function this GO :0043123 and positive regulation of I-kappaB kinase/NF-kappaB signaling and biological process this GO :0043330 and response to exogenous dsRNA and biological process this GO :0044212 and transcription regulatory region DNA binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045351 and type I interferon biosynthetic process and biological process this GO :0045358 and negative regulation of interferon-beta biosynthetic process and biological process this GO :0050689 and negative regulation of defense response to virus by host and biological process this GO :0050715 and positive regulation of cytokine secretion and biological process this GO :0051607 and defense response to virus and biological process this GO :0060333 and interferon-gamma-mediated signaling pathway and biological process this GO :0060337 and type I interferon signaling pathway and biological process this GO :0060340 and positive regulation of type I interferon-mediated signaling pathway and biological process this GO :0071359 and cellular response to dsRNA and biological process this GO :0071888 and macrophage apoptotic process and biological process this GO :0097300 and programmed necrotic cell death and biological process, IRF3 and IDBG-63225 and ENSG00000126456 and 3661, IRF3 and IDBG-640634 and ENSBTAG00000006633 and 516979, Irf3 and IDBG-181123 and ENSMUSG00000003184 and 54131, nuclei, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000975 and regulatory region DNA binding and molecular function this GO :0002224 and toll-like receptor signaling pathway and biological process this GO :0002756 and MyD88-independent toll-like receptor signaling pathway and biological process this GO :0003677 and DNA binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0003712 and transcription cofactor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006355 and regulation of transcription, this GO :0000975 : regulatory region DNA binding, this GO :0000975 : regulatory region DNA binding and also this GO :0003677 : DNA binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0003712 : transcription cofactor activity and also this GO :0005515 : protein binding and also this GO :0042802 : identical protein binding and also this GO :0042803 : protein homodimerization activity and also this GO :0044212 : transcription regulatory region DNA binding, this GO :0003677 : DNA binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0003712 : transcription cofactor activity, this GO :0005515 : protein binding, this GO :0042802 : identical protein binding, this GO :0042803 : protein homodimerization activity, this GO :0044212 : transcription regulatory region DNA binding, transcription regulatory region DNA binding, interferon regulatory factor 3 |
| Identity: |
6118 |
| Gene: |
IRF3 |
More about : IRF3 |
| Long gene name: |
interferon regulatory factor 3 |
| Locus: |
19q13, 33 |
| Discovery year: |
1996-11-13 |
| Entrez gene record: |
3661 |
| Pubmed identfication: |
8524823 |
| RefSeq identity: |
NM_001571 |
| Havana BLAST/BLAT: |
OTTHUMG00000183287 |