| Antigen detectable with this ELISA kit: |
Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
055ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4), The concentration of Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Cytotoxic T-Lymphocyte Associated 4 (CTLA4) |
| Short name: |
Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Cytotoxic T-Lymphocyte Associated protein 4 (cytotoxic T-lymphocyte-associated protein 4) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CD and CD152 and CELIAC3 and CTLA-4 and GRD4 and GSE and IDDM12, CTLA-4 and IDBG-643375 and ENSBTAG00000013170 and 281732, CTLA4 and IDBG-79215 and ENSG00000163599 and 1493, Cell surfaces, Ctla4 and IDBG-159662 and ENSMUSG00000026011 and 12477, protein binding, this GO :0005515 and protein binding and molecular function this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006955 and immune response and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0030889 and negative regulation of B cell proliferation and biological process this GO :0031295 and T cell costimulation and biological process this GO :0042130 and negative regulation of T cell proliferation and biological process this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045334 and clathrin-coated endocytic vesicle and cellular component this GO :0045590 and negative regulation of regulatory T cell differentiation and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0050777 and negative regulation of immune response and biological process this GO :0050853 and B cell receptor signaling pathway and biological process, this GO :0005515 : protein binding, this GO :0005515 : protein binding, cytotoxic T-lymphocyte-associated protein 4 |
| Identity: |
2505 |
| Gene: |
CTLA4 |
More about : CTLA4 |
| Long gene name: |
cytotoxic T-lymphocyte associated protein 4 |
| Synonyms gene: |
CELIAC3 IDDM12 |
| Synonyms gene name: |
celiac disease 3 insulin-dependent diabetes mellitus 12 |
| Synonyms: |
CD152 CD GSE |
| Locus: |
2q33, 2 |
| Discovery year: |
1989-05-25 |
| Entrez gene record: |
1493 |
| Pubmed identfication: |
3220103 8817351 |
| RefSeq identity: |
NM_005214 |
| Classification: |
CD molecules V-set domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000132877 |