| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
c-AblTyr185 |
| Specificity: |
This antibody reacts specifically with c-Abl (Tyr185) |
| Modification: |
Phosphorylation |
| Modification site: |
Tyr185 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human c-Abl isoform a around the phosphorylation site of Tyr185 [KL(p-Y)VS] |
| Gene ID: |
25 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Activity of c-Abl protein is negatively regulated by its SH3 domain, The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, The t(9, [provided by RefSeq], and deletion of the SH3 domain turns ABL1 into an oncogene, and stress response, cell adhesion, cell division, suggesting a cell cycle function for ABL1, with alternatively spliced first exons spliced to the common exons 2-11, 22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia, The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
ABL, ABL1_HUMAN, Abelson Murine Leukemia Viral Oncogene Homolog 1, Abelson murine leukemia viral v abl oncogene homolog 1, Abl 1, Abl protein, Abl1, Bcr/c abl oncogene protein, JTK 7, JTK7, Proto oncogene tyrosine protein kinase ABL1, Transformation gene oncogene ABL, c-Ablphospho Tyr185, p-c-AblTyr185, p150 , tyrosine-protein kinase ABL1 isoform b, v abl, v abl Abelson murine leukemia viral oncogene homolog 1, c-Ablphospho Y185 |
| Also known as: |
c-Abl (Tyr185) Polyclonal Antibody |
| Other name: |
Anti-c-Abl (Tyr185) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
c-Abl (Tyr185) |
| Short name: |
Anti-c-Abl (Tyr185) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-c-Abl (Tyr185) (polyclonal) |
| Alternative technique: |
polyclonals |