| Antigen detectable with this ELISA kit: |
Cytochrome P450 27B1 (CYP27B1) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
0, 1, 10ng/mL, 2, 20ng/mL, 5ng/mL, 25ng/mL, 5ng/mL, 625-40ng/mL The standard curve concentrations used for the ELISA&rsquo, 625ng/mL, s were 40ng/mL |
| Sensitivity: |
235ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 27B1 (CYP27B1), The concentration of Cytochrome P450 27B1 (CYP27B1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 27B1 (CYP27B1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Cytochrome P450 27B1 (CYP27B1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Cytochrome P450 27B1 (CYP27B1) |
| Short name: |
Cytochrome P450 27B1 (CYP27B1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
family 27, polypeptide 1) Enzyme-linked immunosorbent assay reagent, subfamily B, Cytochrome P450 27B1 (cytochrome P450 |
| Alternative technique: |
elisas |
| Alternative to gene target: |
BT, CP2B and CYP1 and CYP1alpha and CYP27B and P450c1 and PDDR and VDD1 and VDDR and VDDRI and VDR, CYP27B1 and IDBG-43755 and ENSG00000111012 and 1594, Cyp27b1 and IDBG-194013 and ENSMUSG00000006724 and 13115, Cytoplasm, acting on paired donors, acting on paired donors, acting on paired donors, family 27, oxidoreductase activity, polypeptide 1, subfamily B, this GO :0004498 and calcidiol 1-monooxygenase activity and molecular function this GO :0005506 and iron ion binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005741 and mitochondrial outer membrane and cellular component this GO :0006766 and vitamin metabolic process and biological process this GO :0006805 and xenobiotic metabolic process and biological process this GO :0006816 and calcium ion transport and biological process this GO :0008202 and steroid metabolic process and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0010956 and negative regulation of calcidiol 1-monooxygenase activity and biological process this GO :0010980 and positive regulation of vitamin D 24-hydroxylase activity and biological process this GO :0016705 and oxidoreductase activity, this GO :0004498 : calcidiol 1-monooxygenase activity, this GO :0004498 : calcidiol 1-monooxygenase activity and also this GO :0005506 : iron ion binding and also this GO :0016705 : oxidoreductase activity, this GO :0005506 : iron ion binding, this GO :0016705 : oxidoreductase activity, this GO :0020037 : heme binding, with incorporation or reduction of molecular oxygen, with incorporation or reduction of molecular oxygen and also this GO :0020037 : heme binding, with incorporation or reduction of molecular oxygen and molecular function this GO :0020037 and heme binding and molecular function this GO :0030282 and bone mineralization and biological process this GO :0030308 and negative regulation of cell growth and biological process this GO :0030500 and regulation of bone mineralization and biological process this GO :0032496 and response to lipopolysaccharide and biological process this GO :0033280 and response to vitamin D and biological process this GO :0034341 and response to interferon-gamma and biological process this GO :0036378 and calcitriol biosynthetic process from calciol and biological process this GO :0042359 and vitamin D metabolic process and biological process this GO :0042369 and vitamin D catabolic process and biological process this GO :0043627 and response to estrogen and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0045618 and positive regulation of keratinocyte differentiation and biological process this GO :0046697 and decidualization and biological process this GO :0055074 and calcium ion homeostasis and biological process this GO :0055114 and oxidation-reduction process and biological process this GO :0070314 and G1 to G0 transition and biological process this GO :0070564 and positive regulation of vitamin D receptor signaling pathway and biological process, 61430 and IDBG-634881 and ENSBTAG00000016906 and 539630, cytochrome P450 |
| Identity: |
2606 |
| Gene: |
CYP27B1 |
More about : CYP27B1 |
| Long gene name: |
cytochrome P450 family 27 subfamily B member 1 |
| Synonyms gene: |
VDD1 PDDR |
| Synonyms gene name: |
cytochrome P450, family 27, polypeptide 1 , polypeptide 1 cytochrome P450, subfamily B, subfamily XXVIIB (25-hydroxyvitamin D-1-alpha-hydroxylase) |
| Synonyms: |
CYP1 P450c1 |
| Synonyms name: |
VDDR I 1alpha(OH)ase 25-Hydroxyvitamin D3 1alpha-hydroxylase |
| Locus: |
12q14, 1 |
| Discovery year: |
1998-03-24 |
| GenBank acession: |
AB006987 |
| Entrez gene record: |
1594 |
| Pubmed identfication: |
9295274 9344864 |
| RefSeq identity: |
NM_000785 |
| Classification: |
Cytochrome P450 family 27 |
| Havana BLAST/BLAT: |
OTTHUMG00000170457 |