| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
GRB10/14 |
| Specificity: |
This antibody reacts specifically with GRB10/14 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human GRB10 |
| Gene ID: |
2887, 2888 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Binds to, Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation, Delays and reduces AKT1 phosphorylation in response to insulin stimulation, GRB14 is a potent inhibitor of insulin-stimulated MAPK3 phosphorylation, May inhibit NEDD4-mediated degradation of KDR/VEGFR-2, May play a role in mediating insulin-stimulated ubiquitination of INSR, Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6, Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, Positive regulator of the KDR/VEGFR-2 signaling pathway, Recruits NEDD4 to IGF1R, The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation, activated receptors tyrosine kinases, and suppress signals from, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors, increased internalization and degradation by both the proteasomal and lysosomal pathways, leading to IGF1R ubiquitination, leading to proteasomal degradation, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal, Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
GRB10/14 Polyclonal Antibody |
| Other name: |
Anti-GRB10/14 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
GRB10/14 |
| Short name: |
Anti-GRB10/14 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-GRB10/14 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
4564 |
| Gene: |
GRB10 |
More about : GRB10 |
| Long gene name: |
growth factor receptor bound protein 10 |
| Synonyms gene name: |
growth factor receptor-bound protein 10 |
| Locus: |
7p12, 1 |
| Discovery year: |
1996-05-30 |
| Entrez gene record: |
2887 |
| RefSeq identity: |
NM_005311 |
| Classification: |
Pleckstrin homology domain containing SH2 domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000150622 |
| Locus Specific Databases: |
LRG_1032 |