| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
BLNK |
| Specificity: |
This antibody reacts specifically with BLNK |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cell membrane, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human BLNK |
| Gene ID: |
29760 |
| Swiss Prot: |
Q8WV28 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
However, Important for the activation of NF-kappa-B and NFAT, MAP kinase p38 and JNK, May be required for the RAC1-JNK pathway, May play an important role in BCR-induced B-cell apoptosis, Modulates AP1 activation, Plays a critical role in orchestrating the pro-B cell to pre-B cell transition, Plays a role in the activation of ERK/EPHB2, Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes, bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling, downstream of the B-cell receptor (BCR), Functions as a central linker protein |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
SLP65 Ly57 SLP-65 BLNK-s BASH bca |
| Also known as: |
BLNK Polyclonal Antibody |
| Other name: |
Anti-BLNK Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
BLNK |
| Short name: |
Anti-BLNK (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-B-cell linker (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
BLNK and IDBG-83724 and ENSG00000095585 and 29760, BT, Blnk and IDBG-165316 and ENSMUSG00000061132 and 17060, Plasma membranes, protein binding, this GO :0005068 and transmembrane receptor protein tyrosine kinase adaptor activity and molecular function this GO :0005070 and SH3/SH2 adaptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006954 and inflammatory response and biological process this GO :0006959 and humoral immune response and biological process this GO :0007169 and transmembrane receptor protein tyrosine kinase signaling pathway and biological process this GO :0009967 and positive regulation of signal transduction and biological process this GO :0030183 and B cell differentiation and biological process this GO :0035556 and intracellular signal transduction and biological process, this GO :0005068 : transmembrane receptor protein tyrosine kinase adaptor activity, this GO :0005068 : transmembrane receptor protein tyrosine kinase adaptor activity and also this GO :0005070 : SH3/SH2 adaptor activity and also this GO :0005515 : protein binding, this GO :0005070 : SH3/SH2 adaptor activity, this GO :0005515 : protein binding, 24520 and IDBG-638042 and ENSBTAG00000021358 and 510393, B-cell linker |
| Identity: |
14211 |
| Gene: |
BLNK |
More about : BLNK |
| Long gene name: |
B-cell linker |
| Synonyms name: |
B-cell adapter containing a SH2 domain protein B-cell activation Src homology [SH2] domain-containing leukocyte protein of 65 kD B cell adaptor containing SH2 domain |
| Locus: |
10q24, 1 |
| Discovery year: |
2001-07-16 |
| GenBank acession: |
AF068180 |
| Entrez gene record: |
29760 |
| Pubmed identfication: |
9697839 10583958 |
| RefSeq identity: |
NM_013314 |
| Classification: |
SH2 domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000018827 |
| Locus Specific Databases: |
BLNKbase: Mutation registry for BLNK deficiency LRG_21 |