| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
DC-SIGN |
| Specificity: |
This antibody reacts specifically with DC-SIGN |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Extracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human DC-SIGN |
| Gene ID: |
30835 |
| Swiss Prot: |
Q9NNX6 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
HCV E2, HIV-2 gp120, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2, On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates, Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, SIV gp120, Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells, The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response, Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments, cytomegalovirus gB, dengue virus gE, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA, ebolavirus glycoproteins, including HIV-1 gp120, mannose in Klebsiella pneumonae LPS, Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
DC-SIGN CDSIGN DC-SIGN1 CLEC4L |
| Also known as: |
DC-SIGN Polyclonal Antibody |
| Other name: |
Anti-DC-SIGN Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
DC-SIGN |
| Short name: |
Anti-DC-SIGN (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-DC-SIGN (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
1641 |
| Gene: |
CD209 |
More about : CD209 |
| Long gene name: |
CD209 molecule |
| Synonyms gene name: |
CD209 antigen |
| Locus: |
19p13, 2 |
| Discovery year: |
2000-07-19 |
| GenBank acession: |
M98457 |
| Entrez gene record: |
30835 |
| Pubmed identfication: |
1518869 |
| RefSeq identity: |
NM_021155 |
| Classification: |
CD molecules Scavenger receptors C-type lectin domain family |
| Havana BLAST/BLAT: |
OTTHUMG00000182530 |